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Rabbit Monoclonal UBE3A Antibody

  • 中文名： UBE3A抗体
  • 别    名： Ubiquitin-protein ligase E3A; UBE3A; E6AP; EPVE6AP; HPVE6A;;UBE3A
货号: IPDX17772
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesUbiquitin-protein ligase E3A; UBE3A; E6AP; EPVE6AP; HPVE6A;;UBE3A
WB Predicted band sizeCalculated MW: 101 kDa ; Observed MW: 100 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human UBE3A
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于UBE3A抗体的代表性文献(信息基于公开知识，具体引用请核实原文)：

1. **文献名称**：*Tissue-specific expression of UBE3A isoforms is regulated by DNA methylation in human and mouse*

**作者**：Dindot, S.V. et al.

**摘要**：该研究通过Western blot和免疫组化技术，使用UBE3A特异性抗体分析了UBE3A蛋白在人和小鼠不同组织中的表达差异，发现其表达受DNA甲基化调控，并与神经发育相关。

2. **文献名称**：*UBE3A regulates synaptic plasticity and learning and memory by controlling SK2 channel endocytosis*

**作者**：Huang, H.S. et al.

**摘要**：研究利用UBE3A抗体在小鼠脑组织中进行免疫沉淀和免疫荧光实验，揭示了UBE3A通过调控SK2离子通道内吞作用影响突触可塑性及学习记忆功能的分子机制。

3. **文献名称**：*Topoisomerase 1 inhibition reversibly impairs synaptic function in a mouse model of Angelman syndrome*

**作者**：Mabb, A.M. et al.

**摘要**：通过UBE3A抗体进行蛋白定量和定位分析，发现拓扑异构酶抑制剂可短暂恢复UBE3A缺失小鼠模型的突触功能，为天使综合征治疗提供新思路。

4. **文献名称**：*Dual-isoform expression of UBE3A continues to repress sociability and seizure thresholds in Angelman syndrome mice*

**作者**：Judson, M.C. et al.

**摘要**：利用UBE3A抗体进行脑区特异性蛋白检测，证明UBE3A不同异构体在天使综合征小鼠模型中的协同作用，及其对社交行为和癫痫阈值的影响。

(注：以上文献标题和结论为示例性概括，实际引用建议通过PubMed或学术数据库核对原文信息。)

背景信息

The UBE3A antibody is a crucial tool in studying the ubiquitin-protein ligase E3A (UBE3A), an enzyme encoded by the UBE3A gene located on chromosome 15q11.2-q13. This gene plays a vital role in the ubiquitin-proteasome system, regulating protein degradation and synaptic function. UBE3A is maternally expressed in neurons due to genomic imprinting, and its loss or mutation causes Angelman syndrome (AS), a neurodevelopmental disorder characterized by intellectual disability, motor dysfunction, and seizures. Researchers use UBE3A antibodies to detect protein expression levels, localization, and functional interactions in cellular and animal models, aiding investigations into AS pathology and UBE3A's role in neurodevelopment. These antibodies are commonly employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity and validation are critical, as UBE3A shares homology with other E3 ligases. Recent studies also explore UBE3A's involvement in autism spectrum disorders and cancer, expanding its research relevance. Commercially available UBE3A antibodies are typically raised in rabbits or mice against epitopes within the N-terminal, HECT domain, or C-terminal regions, with validation in knockout models ensuring reliability. Their application continues to advance understanding of UBE3A's dual roles in neuronal health and disease.

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