| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | protein kinase C beta type；PRKCB；Prkcb；PKC-B; PKC-beta；PRKCB1；PKC Beta-I；EC:2.7.11.13;PKC beta |
| WB Predicted band size | Calculated MW: 77 kDa ; Observed MW: 40,77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKC beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PKCβ1抗体的3篇参考文献示例(内容为模拟虚构，仅供参考)：
1. **文献名称**：*"Protein kinase C β1 activation induces proliferation and angiogenesis in human multiple myeloma cells"*
**作者**：Podar K, et al.
**摘要**：该研究利用PKCβ1特异性抗体证实了PKCβ1在多发性骨髓瘤细胞中的高表达，并发现其激活可促进肿瘤细胞增殖及血管生成，提示其作为治疗靶点的潜力。
2. **文献名称**：*"Role of PKCβ1 in diabetic retinopathy: Insights from a mouse model"*
**作者**：Koya D, et al.
**摘要**：通过免疫组化与Western blot分析(使用PKCβ1抗体)，作者发现高血糖环境下视网膜中PKCβ1活性显著升高，并与血管通透性增加相关，为糖尿病并发症机制提供依据。
3. **文献名称**：*"Differential localization of PKCβ isoforms in neuronal tissues revealed by subtype-specific antibodies"*
**作者**：Saito N, Shimizu H
**摘要**：研究利用PKCβ1和β2亚型特异性抗体，揭示了PKCβ1主要分布于大脑皮层和海马神经元中，提示其在神经信号传导中的独特作用。
**注意**：以上文献信息为示例，实际引用时请核对真实文献的准确性。
The protein kinase C beta 1 (PKCβ1) antibody is a crucial tool for studying the PKC family, a group of serine/threonine kinases involved in diverse cellular signaling pathways. PKCβ1. encoded by the PRKCB gene, is a member of the classical PKC subfamily, activated by calcium, diacylglycerol (DAG), and phospholipids. It plays roles in cell proliferation, differentiation, and survival, particularly in immune cells, neurons, and vascular tissues.
The PKCβ1 antibody is designed to specifically recognize and bind to the PKCβ1 isoform, distinguishing it from the closely related PKCβ2 splice variant. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and activation in tissues or cultured cells. Research has linked PKCβ1 dysregulation to pathologies such as cancer (e.g., promoting tumor angiogenesis and drug resistance), diabetic complications (e.g., retinopathy and nephropathy via hyperglycemia-induced signaling), and inflammatory diseases.
Pharmaceutical targeting of PKCβ1. including inhibitors like ruboxistaurin, has been explored in clinical trials for diabetic complications. The antibody aids in validating target engagement, monitoring expression changes, and elucidating mechanistic pathways in these contexts. Its specificity and reliability make it essential for both basic research and therapeutic development.
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