| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone lysine demethylase PHF8; MRXSSD; PHD finger protein 8; PHF8; ZNF422;;PHF8 |
| WB Predicted band size | Calculated MW: 118 kDa ; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PHF8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PHF8抗体的3篇参考文献，包含文献名称、作者及摘要概要：
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1. **文献名称**：*PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression*
**作者**：Liu, W., Tanasa, B., Tyurina, O., et al.
**摘要**：该研究通过PHF8抗体进行染色质免疫沉淀(ChIP)和免疫印迹分析，发现PHF8作为组蛋白H4K20me1/2的去甲基化酶，调控细胞周期相关基因表达，影响G2/M期转换。
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2. **文献名称**：*PHF8 promotes cell proliferation by regulating cell cycle in colorectal cancer*
**作者**：Wang, J., Chen, Y., Huang, Y., et al.
**摘要**：利用PHF8抗体进行免疫组化实验，发现PHF8在结直肠癌中高表达，通过调控Cyclin D1等基因加速细胞周期进程，促进肿瘤生长，提示其作为潜在治疗靶点。
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3. **文献名称**：*PHF8 mutations cause X-linked mental retardation with facial dysmorphism*
**作者**：Kleine-Kohlbrecher, D., Christensen, J., Vandamme, J., et al.
**摘要**：研究通过PHF8抗体的免疫荧光和Western blot技术，揭示PHF8基因突变导致其组蛋白去甲基化功能丧失，与X连锁智力障碍及面部畸形相关，为神经发育异常机制提供依据。
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以上文献均涉及PHF8抗体在功能研究中的应用，涵盖表观遗传调控、癌症生物学及神经发育疾病等领域。如需具体实验细节或扩展文献，可进一步检索PubMed或Web of Science数据库。
PHF8 (Plant Homeodomain Finger Protein 8) is a member of the JmjC domain-containing histone demethylase family, which plays a critical role in epigenetic regulation by removing methyl groups from specific lysine residues on histones. Primarily localized in the nucleus, PHF8 targets histone H3 lysine 9 (H3K9me2/me1) and H4 lysine 20 (H4K20me1), modulating chromatin structure and gene expression. It is involved in diverse cellular processes, including transcriptional regulation, cell cycle progression, and DNA repair. PHF8 also interacts with transcriptional machinery, such as RNA polymerase II, to influence developmental pathways and neuronal function. Dysregulation of PHF8 has been linked to X-linked intellectual disability, cancer progression, and other disorders, underscoring its importance in both normal physiology and disease.
PHF8 antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies enable researchers to detect PHF8 via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Validated antibodies are critical for ensuring specificity, as cross-reactivity with other JmjC family members can complicate interpretations. Applications include investigating PHF8's role in epigenetic reprogramming, its association with tumorigenesis (e.g., breast cancer, glioblastoma), and its impact on neurodevelopmental disorders. Recent studies also explore PHF8's interaction with non-histone substrates, expanding its regulatory scope. High-quality PHF8 antibodies support mechanistic insights into how epigenetic perturbations contribute to disease, aiding therapeutic target discovery.
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