| WB | 咨询技术 | E.coli |
| IF | 1/20-1/50 | E.coli |
| IHC | 咨询技术 | E.coli |
| ICC | 技术咨询 | E.coli |
| FCM | 咨询技术 | E.coli |
| Elisa | 咨询技术 | E.coli |
| Aliases | groN; nitB; rif; RNA pol beta; RNA polymerase subunit beta; ron; rpoB; stl; stv; tabD; Transcriptase subunit beta;;RNA polymerase beta |
| WB Predicted band size | Calculated MW: 151 kDa ; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | E.coli |
| Immunogen | A synthesized peptide derived from RNA polymerase beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与RNA聚合酶β亚基抗体相关的参考文献摘要概览：
1. **文献名称**: "Production and characterization of monoclonal antibodies against the β subunit of Escherichia coli RNA polymerase"
**作者**: Bergseid M, et al.
**摘要**: 该研究报道了针对大肠杆菌RNA聚合酶β亚基的单克隆抗体制备及特性分析。抗体成功应用于Western blot和免疫沉淀实验，证实其对β亚基的高特异性识别，为研究细菌转录机制提供了工具。
2. **文献名称**: "Antibiotic resistance mutations in the rpoB gene of Mycobacterium tuberculosis alter RNA polymerase stability and function"
**作者**: Telenti A, et al.
**摘要**: 通过抗β亚基抗体(RpoB)检测结核分枝杆菌中rpoB基因突变导致的利福平耐药性。研究揭示了突变如何影响RNA聚合酶结构稳定性，为耐药性快速诊断提供了抗体检测方案。
3. **文献名称**: "Immunological analysis of RNA polymerase II reveals novel phosphorylation-dependent epitopes on the RPB1 and RPB2 subunits"
**作者**: Bregman DB, et al.
**摘要**: 利用抗RNA聚合酶II(RPB1/RPB2)亚基的抗体研究真核生物转录调控。抗体识别β同源亚基(RPB2)的磷酸化表位，揭示了转录延伸阶段的动态修饰机制。
注：RNA聚合酶β亚基在原核生物中称为RpoB，真核生物RNA聚合酶II/III中对应亚基为RPB2/RPC2.上述文献涵盖原核与真核体系，均涉及抗体的实验应用，包括耐药性检测、结构功能分析和翻译后修饰研究。
The RNA polymerase beta (RNAP β) antibody is a crucial tool in molecular biology research, specifically targeting the β subunit of bacterial RNA polymerase. As the core enzyme of transcription in prokaryotes, RNA polymerase consists of multiple subunits (α, β, β', ω), with the β subunit playing a central role in catalytic activity. Encoded by the rpoB gene, the β subunit contributes to the formation of the enzyme's active site, participating in nucleotide substrate binding, RNA chain elongation, and interactions with transcription factors.
This antibody has become particularly significant in studying antibiotic resistance mechanisms. The β subunit serves as the target for rifampicin-class antibiotics, and mutations in rpoB are strongly associated with rifampicin resistance in pathogens like Mycobacterium tuberculosis. Researchers employ RNAP β antibodies in techniques such as Western blotting, immunoprecipitation, and chromatin affinity purification to investigate RNA polymerase assembly, transcriptional regulation, and bacterial stress responses.
In clinical diagnostics, these antibodies aid in detecting RNA polymerase components during bacterial infection studies. Their application extends to evolutionary biology, where conservation of the β subunit across bacterial species allows comparative structural analyses. Recent developments in cryo-EM studies of transcription complexes have further increased demand for high-specificity RNAP β antibodies as structural validation tools. The continued utility of this reagent underscores its importance in both basic microbial physiology research and applied medical microbiology.
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