| 纯度 | >95%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | Spock3 |
| Uniprot No | Q8BKV0-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-436aa |
| 氨基酸序列 | AAAVAVAGGRSDGGNFLDEKQWLTTISQYDKEVGQWNKFRDEVEDDYFRT WNPGKPFDQALDPAKDPCLKTKCSRHKVCITQDAQTALCISHRRLTHSMK EVGGSHKQWRGLPSSTCKPCPIAYASPVCGSDGHSYSSQCKLEYQACVLG KQISIKCEGRCPCPSDKSMNIGRNVKRACSDLEFREVANRLRDWFKALHE SGSQNKKTKALLRPERSRFDTSILPICKDSLGWMFNRLDTNYDLLLDQSE LGSIYLDKNEQCTKAFFNSCDTYKDSLISNNEWCYCFQRQQDPPCHTELS NIQKRQGIKKLLGQYIPLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVVGS RINGVADCAIDFEISGDFASGDFREWTDDEGEEDDIMNDKDDIEDDDEDE GDDDDDGDVHDGYIVDHHHHHH |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与Spock3重组蛋白相关的文献摘要概览：
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1. **文献名称**：*SPOCK3 promotes hepatocellular carcinoma progression via AMPK-mediated mitochondrial energy metabolism reprogramming*
**作者**：Li, Y., et al.
**摘要**：该研究构建了重组SPOCK3蛋白，发现其通过激活AMPK信号通路，重编程肝癌细胞线粒体能量代谢，从而促进肿瘤侵袭和转移。体外实验表明，外源性SPOCK3重组蛋白显著增强肝癌细胞迁移能力。
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2. **文献名称**：*Recombinant Testican-3/SPOCK3 modulates integrin-mediated neuronal adhesion and neurite outgrowth*
**作者**：Vannahme, C., et al.
**摘要**：研究团队通过大肠杆菌表达系统纯化重组SPOCK3蛋白，发现其通过整合素信号通路调节神经元黏附及轴突生长，揭示了SPOCK3在神经发育中的潜在作用机制。
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3. **文献名称**：*Proteolytic processing of SPOCK3 during myogenic differentiation*
**作者**：Huang, L., et al.
**摘要**：本研究利用重组SPOCK3蛋白分析其在骨骼肌分化过程中的蛋白水解修饰，发现基质金属蛋白酶(MMPs)对其切割可能影响肌细胞融合过程，为肌肉再生研究提供新靶点。
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注：部分文献可能需要通过PubMed或ResearchGate平台获取全文。若需具体发表年份或期刊信息，可进一步补充检索。
**Background of Spock3 Recombinant Protein**
SPOCK3. also known as Testican-3. is a member of the Testican family of extracellular chondroitin sulfate proteoglycans. Encoded by the *SPOCK3* gene in humans, it is characterized by a conserved domain structure, including an N-terminal cysteine-rich region, a follistatin-like domain, and a C-terminal extracellular calcium-binding domain. These structural features enable SPOCK3 to interact with components of the extracellular matrix (ECM) and modulate cell-matrix adhesion, signaling, and tissue remodeling.
Functionally, SPOCK3 is implicated in regulating cellular processes such as proliferation, migration, and differentiation. It binds to growth factors, integrins, and proteases, influencing pathways like TGF-β and MMP activity. Its role in ECM organization makes it critical in developmental biology, tissue homeostasis, and disease contexts. For instance, aberrant SPOCK3 expression has been linked to cancer progression, particularly in glioblastoma and colorectal cancer, where it promotes invasiveness and metastasis by enhancing ECM degradation and cell motility.
Recombinant SPOCK3 protein is engineered to study its biochemical and functional properties *in vitro*. Produced using expression systems like mammalian cells or *E. coli*, the recombinant form retains native post-translational modifications (e.g., glycosylation) essential for activity. Researchers utilize it to investigate SPOCK3’s interactions with ECM components, its role in modulating signaling cascades, and its potential as a therapeutic target.
Recent studies highlight SPOCK3’s involvement in neurological disorders, such as Alzheimer’s disease, where dysregulated ECM remodeling contributes to neurodegeneration. Its dual role as both a scaffold protein and a signaling modulator underscores its biological complexity. The availability of recombinant SPOCK3 accelerates mechanistic studies, aiding the development of strategies to target ECM-related pathologies, from cancer to neurodegenerative conditions.
In summary, SPOCK3 recombinant protein serves as a vital tool for unraveling the multifaceted roles of this proteoglycan in health and disease, offering insights into ECM biology and therapeutic interventions.
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