| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TPRH; TRPH |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TPH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NDUFS3抗体的文献示例(虚构内容，供参考格式)：
1. **文献名称**: *Mitochondrial complex I deficiency caused by NDUFS3 mutations: Immunoblot analysis using anti-NDUFS3 antibody*
**作者**: Smith J, et al.
**摘要**: 研究利用NDUFS3特异性抗体，通过Western blot和免疫组化技术，揭示了NDUFS3基因突变导致线粒体复合物I组装缺陷，与儿童Leigh综合征的发病机制相关。
2. **文献名称**: *NDUFS3 as a biomarker in colorectal cancer: Antibody-based detection of protein expression levels*
**作者**: Chen L, et al.
**摘要**: 通过抗NDUFS3抗体的免疫印迹和免疫荧光分析，发现结直肠癌组织中NDUFS3蛋白表达显著下调，提示其可能作为线粒体功能异常的肿瘤标志物。
3. **文献名称**: *Characterization of NDUFS3 knockout mice using specific monoclonal antibodies*
**作者**: García R, et al.
**摘要**: 研究利用抗NDUFS3单克隆抗体，证实NDUFS3敲除小鼠模型中复合物I功能丧失，并导致神经退行性变和运动障碍表型。
4. **文献名称**: *Anti-NDUFS3 autoantibodies in autoimmune encephalitis: A case-control study*
**作者**: Wang Y, et al.
**摘要**: 首次报道在自身免疫性脑炎患者血清中检测到抗NDUFS3自身抗体，提示线粒体蛋白异常免疫反应可能与神经系统疾病相关。
(注：以上为示例模板，实际文献需通过PubMed/Google Scholar等平台检索。)
NDUFS3 (NADH:ubiquinone oxidoreductase core subunit S3) is a critical component of mitochondrial complex I, the largest enzyme in the electron transport chain responsible for oxidizing NADH to NAD+ and transferring electrons to ubiquinone. This 30 kDa nuclear-encoded subunit is located in the hydrophilic matrix arm of complex I and plays a structural role in maintaining the enzyme's stability while participating in electron transfer processes. As part of the core subcomplex, NDUFS3 interacts with other subunits like NDUFS1 and NDUFV1 to form the functional catalytic core.
Antibodies targeting NDUFS3 are essential tools for studying complex I assembly, function, and deficiencies associated with mitochondrial disorders. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression levels, cellular localization, and complex I integrity. Mutations in NDUFS3 have been linked to severe mitochondrial encephalopathies, Leigh syndrome, and other oxidative phosphorylation defects. Researchers also utilize these antibodies to investigate cancer metabolism, neurodegenerative diseases, and aging processes where mitochondrial dysfunction is implicated. Commercial NDUFS3 antibodies are typically raised against specific epitopes in human or mouse orthologs, with validation performed using knockout cell lines or tissue samples from patients with confirmed complex I deficiencies. Proper validation remains crucial due to structural similarities between mitochondrial complex subunits.
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