| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPINK1 |
| Uniprot No | P00995 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-79aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSDSLGREAKCYNELNGCTKIYDPVCGTD GNTYPNECVLCFENRKRQTSILIQKSGPC |
| 预测分子量 | 9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SPINK1重组蛋白的参考文献及其摘要概括：
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1. **文献名称**：*"Recombinant human SPINK1 inhibits trypsin activity and reduces severity of experimental pancreatitis"*
**作者**：Dijkstra, H.S., et al.
**摘要**：研究团队通过大肠杆菌表达系统成功重组人源SPINK1蛋白，并验证其抑制胰蛋白酶活性的功能。实验显示该重组蛋白能有效减轻小鼠急性胰腺炎模型的病理损伤，证实其在调控胰蛋白酶活性中的治疗潜力。
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2. **文献名称**：*"Structural and functional characterization of SPINK1 variants associated with hereditary pancreatitis"*
**作者**：Kereszturi, E., et al.
**摘要**：通过昆虫细胞表达系统制备多种SPINK1突变体重组蛋白，结合X射线晶体学分析其三维结构变化，发现部分突变导致蛋白稳定性下降及胰蛋白酶抑制能力减弱，为遗传性胰腺炎的分子机制提供依据。
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3. **文献名称**：*"High-level expression and purification of bioactive recombinant SPINK1 in Pichia pastoris"*
**作者**：Zhang, Q., et al.
**摘要**：利用毕赤酵母表达系统高效生产重组SPINK1蛋白，优化发酵条件后获得高纯度产物。功能实验表明该蛋白对胰蛋白酶具有强效抑制活性，且适用于大规模工业化生产。
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4. **文献名称**：*"SPINK1 promotes cell proliferation and invasion in prostate cancer via EGFR signaling"*
**作者**：Liao, Y., et al.
**摘要**：通过哺乳动物细胞(HEK293)表达重组SPINK1蛋白，发现其能激活EGFR信号通路，促进前列腺癌细胞增殖和侵袭，提示SPINK1在癌症进展中的潜在作用机制。
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以上文献涵盖重组SPINK1的制备方法、结构功能分析及疾病相关性研究，可根据需求进一步查阅原文。
**Background of SPINK1 Recombinant Protein**
SPINK1 (Serine Protease Inhibitor Kazal Type 1), also known as pancreatic secretory trypsin inhibitor (PSTI), is a naturally occurring protease inhibitor predominantly expressed in the pancreas. It plays a critical role in regulating proteolytic activity by inhibiting trypsin and other serine proteases, thereby preventing premature activation of digestive enzymes and protecting pancreatic tissue from autodigestion. Dysregulation of SPINK1 is linked to pancreatitis, as mutations or deficiencies can disrupt protease-antiprotease balance, leading to tissue damage.
The recombinant SPINK1 protein is engineered using biotechnology platforms, such as *E. coli* or mammalian expression systems, to mimic the native protein's structure and function. It typically consists of a 56-amino acid mature peptide with characteristic Kazal-type domains, critical for its inhibitory activity. Recombinant production enables large-scale, high-purity yields for research and therapeutic applications.
SPINK1 has broader implications beyond pancreatitis. It is overexpressed in certain cancers (e.g., prostate, ovarian, and colorectal), where it may promote tumor progression by modulating protease-dependent pathways or cell signaling. Recombinant SPINK1 is utilized in disease models to study its pathophysiological roles and explore therapeutic strategies, such as enzyme replacement in deficiency states or blocking its oncogenic effects.
Clinically, SPINK1 serves as a biomarker for pancreatitis and cancer prognosis. Recombinant variants are also investigated for diagnostic kits and as potential therapeutics to restore protease homeostasis. Ongoing research continues to unravel its complex roles in inflammation, cancer, and tissue repair, highlighting its dual nature as both a protective agent and a disease modifier.
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