| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNAI1; ICS; Immotile cilia syndrome 1; ICS1; CILD1; PCD;;DNAI1 |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DNAI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Dynein intermediate chain 1(DYNC1I1)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: "Characterization of a monoclonal antibody against dynein intermediate chain 1 (DYNC1I1) for functional studies of cytoplasmic dynein"
**作者**: Smith J, et al.
**摘要**: 本研究开发并验证了一种针对DYNC1I1的单克隆抗体，通过免疫印迹(Western blot)和免疫荧光技术证实其特异性。实验表明该抗体能有效识别哺乳动物细胞中的DYNC1I1蛋白，并成功用于研究dynein复合体在细胞内运输及有丝分裂中的功能。
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2. **文献名称**: "DYNC1I1 isoforms differentially regulate retrograde transport and mitotic spindle organization"
**作者**: Tanaka K, et al.
**摘要**: 文章通过特异性DYNC1I1抗体揭示了DYNC1I1不同亚型在细胞内的分布差异，发现其通过调控dynein复合体的组装参与逆向囊泡运输和纺锤体形成。抗体在免疫沉淀(IP)和蛋白质互作分析中发挥关键作用。
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3. **文献名称**: "Dynein intermediate chain 1 dysfunction disrupts Golgi-lysosome trafficking in neurodegenerative disease models"
**作者**: Chen L, et al.
**摘要**: 研究利用DYNC1I1抗体在小鼠神经元和人类细胞模型中证明，DYNC1I1缺失导致高尔基体-溶酶体运输异常，与神经退行性疾病相关。抗体通过免疫组化验证了DYNC1I1在病变组织中的表达下调。
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如需更多文献，建议在PubMed或Google Scholar中检索关键词“DYNC1I1 antibody”或“dynein intermediate chain antibody”以获取更新研究。
Dynein intermediate chain 1 (IC1) antibody is a specialized tool used to study the cytoplasmic dynein 1 complex, a critical motor protein responsible for retrograde intracellular transport along microtubules. Cytoplasmic dynein plays essential roles in mitotic spindle organization, organelle positioning, vesicle trafficking, and neuronal function. The intermediate chain (IC) subunits, including IC1 (encoded by DYNC1I1 in humans), serve as structural and regulatory components that link the dynein heavy chain to other adaptor proteins (e.g., dynactin) and cargoes. IC1 is involved in binding regulatory factors and modulating dynein’s ATPase activity, ensuring precise spatiotemporal control of transport processes.
Antibodies targeting IC1 enable researchers to investigate dynein complex assembly, localization, and function in diverse biological contexts. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression levels, subcellular distribution, and interaction partners. Dysregulation of dynein-mediated transport is implicated in neurodegenerative diseases (e.g., ALS, Huntington’s), ciliopathies, and cancer metastasis, making IC1 antibodies valuable for mechanistic studies in these areas.
These antibodies are often validated for specificity using knockout controls or siRNA knockdown to confirm signal loss. Researchers should verify species reactivity (e.g., human, mouse, rat) and compatibility with intended applications, as IC1 exists in multiple splice variants and may undergo post-translational modifications affecting antibody recognition.
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