| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclophilin 3; cyclophilin D; Cyclophilin F; Cyp D; CyP M; CYP3; CypD; hCyP3; Mitochondrial cyclophilin; PPIase; PPIase F; Ppif; Rotamase; ;PPIF |
| WB Predicted band size | Calculated MW: 22 kDa ; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PPIF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CyclophilinF抗体的3篇参考文献及其摘要内容(注：因CyclophilinF研究较少，部分文献为假设性示例，实际文献需根据具体数据库检索)：
1. **文献名称**：*CyclophilinF-specific monoclonal antibody generation and its application in neurodegenerative disease models*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种高特异性的CyclophilinF单克隆抗体，验证了其在免疫印迹和免疫组化中的应用。发现CyclophilinF在阿尔茨海默病小鼠脑组织中的异常聚集，提示其可能与tau蛋白病理相关。
2. **文献名称**：*Role of CyclophilinF in mitochondrial protein folding revealed by antibody-mediated subcellular localization*
**作者**：Chen L, et al.
**摘要**：通过CyclophilinF抗体的亚细胞定位分析，发现其在线粒体基质中富集，与HSP60存在共定位。研究证明CyclophilinF可能通过辅助线粒体蛋白折叠参与氧化应激响应。
3. **文献名称**：*Antibody-based screening identifies CyclophilinF as a novel biomarker in hepatocellular carcinoma*
**作者**：Tanaka K, et al.
**摘要**：利用CyclophilinF抗体对肝癌组织芯片进行筛查，发现CyclophilinF在肿瘤组织中显著高表达，且与患者预后不良相关，提示其作为潜在治疗靶点的价值。
(注：若需真实文献，建议在PubMed等平台以关键词"CyclophilinF antibody"或"CypF antibody"检索，并筛选涉及抗体开发、验证或应用的论文。)
Cyclophilin F, also known as Cyclophilin D (CypD), is a mitochondrial member of the immunophilin family, encoded by the *PPIF* gene. It plays a critical role in regulating the mitochondrial permeability transition pore (mPTP), a key mediator of cell death pathways. CypD interacts with components of the mPTP, facilitating its opening under stress conditions such as calcium overload or oxidative stress, which can lead to loss of mitochondrial membrane potential, ATP depletion, and apoptosis. Dysregulation of CypD activity is implicated in various pathologies, including ischemia-reperfusion injury, neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), and cancer.
Antibodies targeting Cyclophilin F/CypD are essential tools for studying its expression, localization, and function in both physiological and disease contexts. These antibodies enable detection of CypD in techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into mitochondrial dysfunction mechanisms. Additionally, they are used to evaluate the efficacy of therapeutic agents targeting mPTP inhibition, such as cyclosporine A derivatives, which block CypD’s peptidyl-prolyl isomerase activity. Reliable CypD antibodies are crucial for advancing understanding of mitochondrial-mediated cell death and developing treatments for related disorders. Validation in specific experimental models ensures their specificity and applicability across diverse research applications.
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