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产品描述：人白介素35(IL-35)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的IL-35含量。IL-35是一种具有重要生物学意义的靶点。它是一种免疫抑制性细胞因子。研究机制方面，IL-35可调节免疫细胞功能，抑制炎症反应。在多种疾病背景下，如自身免疫病、肿瘤等，对其深入研究有望为开发新的治疗策略提供方向。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域中对IL - 35表达水平的定量分析，例如探索炎症性疾病、自身免疫疾病模型中的免疫调控机制，或研究肿瘤微环境中免疫抑制性细胞因子的动态变化，为研究IL - 35在免疫稳态维持、慢性炎症及肿瘤免疫逃逸中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-35
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-109 | |||||
回收率：
| The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.8722.7522.8122.705 20002.2792.1792.2292.122 10001.5451.5891.5671.460 5000.9521.0020.9770.870 2500.5310.5750.5530.446 1250.3410.3660.3540.247 62.50.2550.2750.2650.158 00.1080.1050.107 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-35 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled IL-35 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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