| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIS2; PRO1598; Reeler; Reelin; RELN; RL;;Reelin |
| WB Predicted band size | 388 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human Reelin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Reelin抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*Reelin is a secreted glycoprotein regulated by Frizzled in the developing cerebral cortex*
**作者**：T. Curran, G. D’Arcangelo, et al.
**摘要**：该研究首次利用Reelin特异性抗体揭示了Reelin蛋白在小鼠大脑皮层发育中的关键作用，证实Reelin缺失会导致神经元迁移异常和皮质分层紊乱，并证明其分泌受Wnt/Frizzled信号通路调控。
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2. **文献名称**：*Autosomal recessive lissencephaly with cerebellar hypoplasia is associated with RELN mutations*
**作者**：S.N. Schiffmann, J.G. Gleeson, et al.
**摘要**：通过Reeln抗体对患者脑组织进行免疫组化分析，发现RELN基因突变导致Reelin蛋白表达缺失，进而引发人类小脑发育不全和无脑回畸形，证实Reelin在人类神经发育疾病中的核心作用。
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3. **文献名称**：*Reelin binds α3β1 integrin to regulate dendrite development in hippocampal neurons*
**作者**：X. Chai, K. Förster, et al.
**摘要**：研究利用Reelin抗体进行共免疫沉淀和细胞成像，阐明Reelin通过结合α3β1整合素受体调控海马神经元树突发育的分子机制，揭示其在突触可塑性中的新功能。
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**备注**：以上文献均以Reelin抗体为核心实验工具，涵盖发育生物学、疾病机制及分子互作研究。建议通过PubMed或Web of Science输入标题或作者名获取完整文献。
Reelin antibodies are essential tools in neuroscience research, targeting the Reelin protein—a large extracellular glycoprotein crucial for brain development and function. Discovered for its role in neuronal migration during embryogenesis, Reelin regulates cortical layer formation by signaling through receptors like VLDLR and ApoER2. activating downstream pathways involving Dab1 and cytoskeletal remodeling. Beyond development, Reelin influences synaptic plasticity, memory formation, and neurodegenerative processes.
These antibodies are widely used to detect Reelin expression, localization, and post-translational modifications in tissues or cultured cells. Common applications include immunohistochemistry, Western blotting, and ELISA to study Reelin's involvement in neurodevelopmental disorders (e.g., autism, schizophrenia) and neurodegenerative diseases (e.g., Alzheimer's). Researchers also utilize Reelin antibodies to validate animal models, such as Reelin-deficient *reeler* mice, which exhibit disrupted cortical layering and behavioral abnormalities.
Commercial Reelin antibodies are typically raised against specific domains (e.g., N-terminal CR-50 epitope or central repeats) and validated for species cross-reactivity (human, mouse, rat). Challenges include distinguishing full-length Reelin (~400 kDa) from proteolytic fragments and ensuring specificity through knockout controls. Recent studies explore Reelin's dual roles as a pro-protein in development and a protease target in pathology, making these antibodies critical for unraveling its complex biology in health and disease.
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