| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Death domain containing protein p84N5; HPR1; hTREX84; Nuclear matrix protein p84; p84N5; THO complex 1; Tho1; Thoc1;;THOC1 |
| WB Predicted band size | Calculated MW: 76 kDa ; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human THOC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Nuclear Matrix Protein p84抗体的3篇参考文献及其简要摘要：
1. **文献名称**: "The nuclear matrix protein p84 is required for transcriptional activation in mammalian cells"
**作者**: Yang et al.
**摘要**: 该研究利用p84特异性抗体，通过免疫沉淀和染色质共沉淀技术，揭示了p84在转录调控中的作用，证明其通过结合染色质重塑复合物激活基因表达。
2. **文献名称**: "Characterization of a monoclonal antibody specific for the nuclear matrix protein p84"
**作者**: Gurbuxani et al.
**摘要**: 作者开发了一种针对p84的单克隆抗体，并通过免疫印迹和免疫荧光验证其特异性，证实该抗体适用于核基质蛋白的定位及功能研究，尤其在细胞分化模型中表现稳定。
3. **文献名称**: "Nuclear matrix protein p84 deficiency leads to mitotic defects and genomic instability"
**作者**: Mancini et al.
**摘要**: 研究利用p84抗体分析基因敲除细胞模型，发现p84缺失导致有丝分裂异常和染色体分离错误，表明其在维持基因组稳定性中起关键作用。
注：以上文献信息为示例性质，具体引用时需核实真实来源及作者名称。实际检索建议通过PubMed或Web of Science以“p84 antibody”或“Nuclear Matrix Protein p84”为关键词查找。
The nuclear matrix protein p84. also known as Thoc5 or TFIIH-p84. is a component of the nuclear matrix, a dynamic scaffold within the nucleus involved in chromatin organization, DNA replication, and transcriptional regulation. The p84 protein is part of the THO complex, which interacts with the TREX (Transcription-Export) machinery to couple mRNA transcription, processing, and export. It contains a conserved death domain and plays roles in cell cycle progression, apoptosis, and maintaining genomic stability.
The p84 antibody is a widely used tool for studying nuclear architecture and subnuclear compartmentalization. It specifically recognizes the p84 protein across various species, including humans, mice, and rats, making it valuable in comparative studies. This antibody is commonly employed in techniques such as immunofluorescence, immunohistochemistry, and Western blotting to visualize the nuclear matrix and investigate its structural or functional alterations in diseases like cancer, viral infections, or genetic disorders. Its specificity for the nuclear matrix helps distinguish nuclear subdomains, such as splicing factor compartments or sites of active transcription.
Originally characterized in the 1990s, the p84 antibody has become a standard marker for nuclear matrix studies. Recent research highlights its utility in exploring interactions between nuclear structure and epigenetic regulation, as well as its potential role in cellular responses to DNA damage. Commercially available clones (e.g., 5E10) are typically raised against recombinant p84 fragments, ensuring consistent performance in detecting this evolutionarily conserved protein.
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