关键信息
-
基因名
OSTM1
-
简介
The OSTM1 protein is indispensable for the maturation and function of osteoclasts and melanocytes. It forms a heteromer with CLCN7, with CLCN7 as the α subunit and OSTM1 as the β subunit. OSTM1 Protein, Human (HEK293, His) is the recombinant human-derived OSTM1 protein, expressed by HEK293 , with C-His labeled tag.
- 应用
-
别名
Osteopetrosis-associated transmembrane protein 1; Chloride channel 7 beta subunit; GL
-
种属
Human
-
表达系统
HEK293
-
标签
C-His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q86WC4
-
表达区间
A32-P284
-
氨基酸序列
ALPFGSSPHRVFHDLLSEQQLLEVEDLSLSLLQGGGLGPLSLPPDLPDLDPECRELLLDFANSSAELTGCLVRSARPVRLCQTCYPLFQQVVSKMDNISRAAGNTSESQSCARSLLMADRMQIVVILSEFFNTTWQEANCANCLTNNSEELSNSTVYFLNLFNHTLTCFEHNLQGNAHSLLQTKNYSEVCKNCREAYKTLSSLYSEMQKMNELENKAEPGTHLCIDVEDAMNITRKLWSRTFNCSVPCSDTVP
-
蛋白长度
Lumenal Domain
-
分子量
32-60 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
OSTM1 (Oligosaccharyltransferase Multimerization 1) is a crucial protein involved in the process of glycosylation, specifically in the transfer of oligosaccharides to nascent proteins within the endoplasmic reticulum. This process is essential for proper protein folding, stability, and function, making OSTM1 significant for cellular homeostasis and overall organismal health. Mutations or dysregulation of OSTM1 have been implicated in various diseases, including certain forms of cancer and congenital disorders. Moreover, its role in the endoplasmic reticulum-associated degradation (ERAD) pathway highlights its importance in maintaining cellular quality control mechanisms. Research on OSTM1 recombinant proteins focuses on understanding its structural dynamics, interaction with glycosylation machinery, and functional implications in disease contexts. By producing OSTM1 as a recombinant protein, researchers aim to elucidate its biochemical properties, regulatory mechanisms, and potential therapeutic targets. This study not only contributes to the fundamental knowledge of glycosylation processes but also paves the way for the development of innovative strategies for treating diseases linked to OSTM1 dysregulation. Through advanced techniques like crystallography and NMR, insights gained from OSTM1 studies can revolutionize our understanding of protein maturation and stability, leading to novel approaches in biomedicine and biotechnology.
