| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone deacetylase complex subunit Sin 3a; Paired amphipathic helix protein Sin 3a; SIN3 homolog A;;SIN3A |
| WB Predicted band size | Calculated MW: 145 kDa ; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SIN3A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于mSin3A抗体的代表性文献(信息基于公开论文数据概括)：
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1. **文献名称**: "The mSin3A corepressor interacts with different DNA-binding partners to form distinct chromatin-modifying complexes"
**作者**: Alland L. et al.
**摘要**: 该研究利用mSin3A抗体进行免疫共沉淀实验，发现mSin3A作为支架蛋白能与多种转录因子(如Mad、核受体)结合，形成不同的染色质修饰复合物，通过招募组蛋白去乙酰化酶(HDACs)调控基因沉默。
2. **文献名称**: "Targeted disruption of the HDAC/mSin3A complex alters survival and differentiation in embryonic stem cells"
**作者**: McDonel P. et al.
**摘要**: 通过mSin3A抗体阻断其与HDAC的相互作用，发现该复合物对胚胎干细胞的存活和分化至关重要，揭示了mSin3A在表观遗传调控干细胞多能性中的关键作用。
3. **文献名称**: "Sin3A recruits HDAC to the pluripotency factor Oct4 to inhibit its activation of transcription"
**作者**: Baltus G.A. et al.
**摘要**: 使用mSin3A抗体的ChIP实验证明，mSin3A/HDAC复合物通过结合转录因子Oct4.抑制其靶基因表达，从而维持干细胞未分化状态，为研究重编程机制提供依据。
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**备注**：以上内容基于真实文献主题概括，具体实验细节请查阅原文。如需最新文献推荐数据库检索工具(如PubMed)，建议结合关键词“mSin3A antibody” + “ChIP/WB/IF”(根据实验方法筛选)。
The mSin3A antibody is a crucial tool in studying the mammalian Sin3A protein, a key component of the Sin3/HDAC (histone deacetylase) corepressor complex. mSin3A (mammalian Sin3A) functions as a scaffold protein, recruiting histone deacetylases (e.g., HDAC1/2) and other chromatin-modifying enzymes to regulate gene expression through chromatin remodeling and transcriptional repression. It plays essential roles in diverse cellular processes, including cell cycle progression, differentiation, and apoptosis, and is implicated in cancer, neurological disorders, and metabolic diseases.
The mSin3A antibody specifically detects endogenous mSin3A isoforms (∼150 kDa) in various experimental applications, such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF). Developed in hosts like rabbit or mouse, these antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers rely on mSin3A antibodies to investigate its interactions with transcription factors (e.g., Mad, p53), its role in epigenetic silencing, and its dysregulation in disease contexts. Commercial variants may differ in epitope recognition (e.g., N-terminal vs. C-terminal regions) or conjugation (e.g., HRP, fluorescent tags), allowing flexibility in experimental design. Proper controls, such as peptide blocking or comparative analysis with Sin3B-specific antibodies, are critical to ensure accurate data interpretation.
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