| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RIG-I-like receptor 1; RLR-1; RLR1; Retinoic acid-inducible gene 1 protein; RIG-1; RIG1; RIG-I; RIGI;;RIGI |
| WB Predicted band size | 107 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human RIGI |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DDX58(RIG-I)抗体的3篇参考文献及其摘要概括：
1. **《RIG-I-mediated antiviral responses to polyinosinic-polycytidylic acid are attenuated by the melanoma differentiation-associated gene 5》**
- 作者：Yoneyama, M. 等(2004)
- 摘要：该研究首次鉴定了DDX58(RIG-I)作为病毒RNA的关键识别受体，并揭示其通过MAVS信号通路激活I型干扰素应答。文中使用抗DDX58抗体验证了其在病毒RNA识别中的功能，为后续抗病毒免疫机制研究奠定基础。
2. **《MAVS forms functional prion-like aggregates to activate and propagate antiviral innate immune response》**
- 作者：Hou, F. 等(2011)
- 摘要：本文发现MAVS蛋白在抗病毒信号中形成朊病毒样聚集体，并依赖DDX58的激活。研究通过Western blot和免疫共沉淀(使用DDX58抗体)证实了DDX58与MAVS的相互作用，阐明其在信号转导中的关键作用。
3. **《Autoimmunity initiates in nonhematopoietic cells and progresses via lymphocytes in an interferon-dependent innate immune pathway》**
- 作者：Sato, S. 等(2018)
- 摘要：该研究探讨了自身免疫疾病中DDX58的异常激活机制，通过免疫组化(IHC)和流式细胞术(使用DDX58特异性抗体)发现，非造血细胞中DDX58的持续活化可触发系统性自身免疫反应，提示其作为潜在治疗靶点。
4. **《Recognition of 5' triphosphate by RIG-I: A mechanistic insight into immunity to RNA viruses》**
- 作者：Rehwinkel, J. 等(2010)
- 摘要：研究解析了DDX58识别病毒5'三磷酸RNA的结构基础，并通过免疫印迹(使用抗DDX58抗体)验证其在不同细胞模型中的表达及激活状态，揭示了其在区分宿主与病毒RNA中的关键作用。
这些文献涵盖了DDX58抗体的实验应用(如Western blot、免疫共沉淀、IHC)，并关联其抗病毒机制与疾病研究，可为相关实验设计提供参考。
**Background of DDX58 Antibody**
DDX58. also known as retinoic acid-inducible gene I (RIG-I), is a cytosolic RNA helicase that acts as a key pattern recognition receptor (PRR) in the innate immune system. It detects viral RNA, particularly short double-stranded RNA (dsRNA) with 5'-triphosphate ends, triggering antiviral signaling pathways. Upon activation, DDX58 interacts with mitochondrial antiviral-signaling protein (MAVS), initiating a cascade that leads to the production of type I interferons (IFNs) and pro-inflammatory cytokines, essential for combating viral infections.
Antibodies targeting DDX58 are critical tools for studying its expression, localization, and function in immune responses. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate DDX58's role in viral detection, autoimmune disorders (e.g., Aicardi-Goutières syndrome), and cancer. Research has highlighted DDX58's dual role: while it protects against viruses, dysregulation can drive chronic inflammation or immune evasion.
DDX58 antibodies often target specific domains, such as the C-terminal regulatory domain or the helicase domain, to probe conformational changes during activation. Mutations or polymorphisms in DDX58 are linked to altered immune responses, making these antibodies valuable in diagnostic and therapeutic research. Overall, DDX58 antibodies enhance understanding of host-pathogen interactions and inform strategies to modulate immune signaling in disease contexts.
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