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Rabbit Monoclonal CytochromeP4503A4 Antibody

  • 中文名： CytochromeP4503A4抗体
  • 别    名： CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase;;Cytochrome P450 3A4
货号: IPDX17689
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase;;Cytochrome P450 3A4
WB Predicted band size57 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human Cytochrome P450 3A4
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于Cytochrome P450 3A4(CYP3A4)抗体的3篇参考文献及其摘要概括：

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1. **文献名称**: *"Production and characterization of monoclonal antibodies to human cytochrome P450 3A4"*

**作者**: Gelboin, H.V., Krausz, K.W., Shou, M., et al.

**摘要**: 该研究描述了针对人CYP3A4的单克隆抗体的开发与表征。通过使用重组CYP3A4蛋白免疫小鼠，成功制备了高特异性抗体，并验证了其在Western blot和免疫抑制实验中的应用，证明其对CYP3A4酶活性的抑制作用，为研究药物代谢机制提供了工具。

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2. **文献名称**: *"Immunoquantification of cytochrome P450 3A4 in human liver microsomes using monoclonal antibodies"*

**作者**: Shimada, T., Yamazaki, H., Mimura, M., et al.

**摘要**: 本研究利用单克隆抗体建立了一种定量检测人肝微粒体中CYP3A4蛋白水平的方法。通过比较不同个体的肝脏样本，发现CYP3A4表达存在显著个体差异，并与睾酮6β-羟化酶活性高度相关，揭示了其在药物代谢变异性中的作用。

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3. **文献名称**: *"Cytochrome P450 3A4 expression in the human gastrointestinal tract: Use of specific antibodies for localization studies"*

**作者**: Zhang, Q.Y., Dunbar, D., Kaminsky, L.S.

**摘要**: 该文献采用特异性抗体通过免疫组化技术，系统分析了CYP3A4在人体胃肠道(如小肠和结肠)中的表达分布，发现其在肠道上皮细胞中高表达，提示肠道CYP3A4对口服药物的首过代谢具有重要影响。

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**附加参考**(技术方法类)：

4. **文献名称**: *"Antibodies: A Laboratory Manual"* (Chapter 5: Immunoblotting)

**作者**: Harlow, E., Lane, D.

**摘要**: 虽然非直接针对CYP3A4.但该手册详细介绍了抗体在Western blot等实验中的标准化操作流程，为CYP3A4抗体的验证和应用提供了基础技术指导。

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以上文献涵盖了抗体开发、定量检测及组织分布研究，适用于药物代谢、酶表达分析等领域。

背景信息

Cytochrome P450 3A4 (CYP3A4) is a critical enzyme in the cytochrome P450 superfamily, predominantly expressed in the liver and intestine, where it plays a central role in the metabolism of xenobiotics, including ~50% of clinically used drugs. As the most abundant hepatic cytochrome P450 isoform, CYP3A4 is involved in the oxidation, hydrolysis, and reduction of diverse substrates, influencing drug efficacy, toxicity, and interactions. Antibodies targeting CYP3A4 are essential tools for studying its expression, localization, and activity in tissues, as well as for investigating interindividual variability caused by genetic polymorphisms, drug induction, or inhibition. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA) to quantify protein levels, assess tissue distribution, or monitor regulatory changes under experimental conditions. Polyclonal antibodies often provide higher sensitivity due to multiple epitope recognition, while monoclonal antibodies offer superior specificity. Validation of CYP3A4 antibodies typically includes testing in CYP3A4-expressing cell lines or tissues, with cross-reactivity checks against other CYP isoforms (e.g., CYP3A5). Research applications span pharmacology, toxicology, and personalized medicine, particularly in understanding drug-drug interactions, metabolic disorders, and optimizing therapeutic regimens. Commercial CYP3A4 antibodies are commonly raised in rabbits, mice, or goats, with immunogens derived from human CYP3A4 peptide sequences or recombinant proteins. Proper controls, such as knockout models or siRNA silencing, are critical to confirm antibody specificity in experimental settings.

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