| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5-HT-1C; 5-HT-2C; 5-HT1C; 5-HT2C; 5-HTR2C; 5HT1C; 5HT2C; 5HTR2C; 5Hydroxytryptamine 2C receptor; Htr1c; HTR2C; ;5 HT2C |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human 5 HT2C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于5HT2C受体抗体的3篇文献示例(注：文献信息为虚构，仅用于示例，实际引用需查询真实数据库)：
1. **文献名称**：*Characterization of a novel monoclonal antibody for 5-HT2C receptor localization in rodent brain*
**作者**：Smith, J. et al.
**摘要**：本研究开发了一种特异性识别5-HT2C受体的单克隆抗体，通过免疫组化和Western blot验证其在啮齿类动物脑组织中的特异性标记，揭示了受体在伏隔核和前额叶皮层的分布特征。
2. **文献名称**：*5-HT2C receptor antibody-based detection in human postmortem tissues: Implications for depression research*
**作者**：Lee, H. & Garcia, R.
**摘要**：利用5-HT2C受体特异性抗体分析抑郁症患者尸检脑组织，发现前扣带回皮层受体表达显著下调，提示其与情绪调节通路的潜在关联。
3. **文献名称**：*Validation of a commercial 5-HT2C receptor antibody for pharmacological studies*
**作者**：Zhang, Q. et al.
**摘要**：系统验证了某商业抗体的特异性及适用性，证明其可用于流式细胞术和免疫荧光，并成功应用于药物诱导受体内化机制的体外研究。
建议通过PubMed或Google Scholar搜索真实文献(关键词如“5-HT2C receptor antibody validation”或“5-HT2C receptor immunohistochemistry”)。
The 5-HT2C receptor (5-hydroxytryptamine 2C receptor), a G protein-coupled receptor (GPCR), is widely expressed in the central nervous system and modulates neurotransmission, mood, appetite, and anxiety. It belongs to the serotonin receptor family and signals primarily through Gq/11 proteins, influencing intracellular calcium release. Dysregulation of 5-HT2C is implicated in psychiatric disorders like depression, schizophrenia, and drug addiction, making it a therapeutic target.
5-HT2C receptor antibodies are essential tools for studying its expression, localization, and function. These antibodies are typically developed against specific epitopes, such as extracellular loops or intracellular domains, and validated for applications like Western blotting, immunohistochemistry, and flow cytometry. High specificity is critical due to structural similarities with other 5-HT2 receptor subtypes (e.g., 5-HT2A/2B). Researchers use these antibodies to explore receptor density changes in disease models, track post-translational modifications (e.g., RNA editing), or evaluate drug-receptor interactions. Some antibodies also distinguish between edited and unedited isoforms, which exhibit distinct signaling properties. Cross-reactivity testing in knockout models ensures reliability. Commercial antibodies often target human, rat, or mouse 5-HT2C receptors, enabling translational studies. Their applications span neuropharmacology, behavioral research, and drug development, particularly for antipsychotics and antidepressants. Quality validation (e.g., siRNA knockdown controls) remains vital to avoid experimental artifacts.
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