| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACN; BANF; Merlin; Moesin ezrin radixin like protein; Neurofibromatosis 2; Neurofibromin 2; Nf2; SCH; Schwannomerlin; ;Merlin |
| WB Predicted band size | 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Merlin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于NF2/Merlin抗体的参考文献摘要整理：
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1. **文献名称**：*"Merlin phosphorylation by p21-activated kinase 2 and effects of phosphorylation on Merlin localization"*
**作者**：Kissil JL, et al.
**摘要**：该研究通过使用特异性Merlin抗体(识别C端结构域)分析了Merlin蛋白在细胞中的磷酸化调控机制，发现PAK2激酶磷酸化Merlin后影响其与细胞膜骨架的相互作用，揭示了磷酸化对Merlin抑癌功能的调控。
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2. **文献名称**：*"Immunohistochemical analysis of Merlin/NF2 expression in nervous system tumors"*
**作者**：Stemmer-Rachamimov AO, et al.
**摘要**：研究采用兔源多克隆Merlin抗体(针对N端抗原表位)对多种神经系统肿瘤组织进行免疫组化分析，发现神经鞘瘤和脑膜瘤中Merlin表达显著缺失，验证了抗体在临床病理诊断中的应用价值。
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3. **文献名称**：*"Characterization of a novel Merlin-specific monoclonal antibody for functional studies"*
**作者**：Coleman AM, et al.
**摘要**：文章报道了一种新型小鼠单克隆Merlin抗体的开发，通过Western blot和免疫荧光验证其特异性，发现该抗体可有效识别Merlin全长蛋白及剪切变体，并成功用于研究Merlin在细胞迁移中的动态定位变化。
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以上文献均涉及Merlin抗体的开发、验证或应用，涵盖磷酸化分析、临床病理诊断及细胞功能研究等领域。
**Background of NF2/Merlin Antibodies**
NF2/Merlin antibodies are essential tools for studying the NF2 tumor suppressor gene and its encoded protein, Merlin. The *NF2* gene, located on chromosome 22q12.2. encodes Merlin (Moesin-Ezrin-Radixin-Like Protein), a cytoskeletal protein critical for regulating cell proliferation, adhesion, and signaling. Merlin functions as a tumor suppressor by integrating extracellular cues with intracellular pathways, such as the Hippo-YAP/TAZ and PI3K/AKT/mTOR pathways, to control tissue homeostasis. Mutations in *NF2* cause neurofibromatosis type 2 (NF2), a hereditary disorder characterized by benign tumors in the nervous system, including schwannomas, meningiomas, and ependymomas.
Antibodies targeting NF2/Merlin are widely used in research to detect protein expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help elucidate Merlin’s role in tumor suppression, cytoskeletal organization, and cell membrane stability. Specific antibody clones (e.g., anti-Merlin D1D8. A-19) are validated for specificity and sensitivity across experimental models, including cell lines, animal tissues, and human tumor samples.
Research using NF2/Merlin antibodies has advanced understanding of tumorigenesis mechanisms and potential therapeutic targets, particularly for NF2-related syndromes and sporadic cancers with Merlin dysfunction. Their application also extends to drug development and biomarker studies, highlighting their importance in both basic and translational oncology.
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