| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ECP51; Reptin 52; REPTIN; RUVB2; RUVBL2; RVB2; TAP54-beta; TIH2; TIP48; TIP49b; zreptin;;RUVBL2 |
| WB Predicted band size | Calculated MW: 51 kDa ; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human RUVBL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇与Reptin/RUVBL2抗体相关的代表性文献(信息经简化整理)：
1. **文献名称**: "RUVBL2 regulates tumorigenesis through transcriptional and metabolic pathways in squamous cell carcinoma"
**作者**: Li Y, et al.
**摘要**: 该研究利用抗RUVBL2抗体进行Western blot和免疫组化分析，发现RUVBL2在鳞状细胞癌中过表达，并通过调控HIF-1α信号通路促进肿瘤代谢重编程和转移。
2. **文献名称**: "Reptin drives chromatin remodeling in DNA damage response via histone variant H2A.Z deposition"
**作者**: Martinez S, et al.
**摘要**: 通过抗Reptin抗体的ChIP-seq实验，揭示了Reptin复合物在DNA损伤修复中通过调控H2A.Z组蛋白变体沉积介导染色质重构，影响同源重组修复效率。
3. **文献名称**: "Targeting RUVBL2-PPIE interaction as a novel therapeutic strategy in glioblastoma"
**作者**: Chen X, et al.
**摘要**: 使用RUVBL2特异性抗体进行免疫共沉淀(Co-IP)鉴定了其与肽基脯氨酰异构酶PPIE的相互作用，证明该互作通过激活Wnt/β-catenin通路促进胶质母细胞瘤干性维持。
4. **文献名称**: "RUVBL2 coordinates β-catenin nuclear transport during intestinal regeneration"
**作者**: Goto N, et al.
**摘要**: 通过免疫荧光结合RUVBL2抗体定位分析，发现该蛋白通过调控β-catenin的核质穿梭影响肠道干细胞增殖，为肠道损伤修复机制提供新见解。
注：以上为模拟文献案例，实际引用需核对具体文献数据库(如PubMed)。建议通过关键词"RUVBL2 antibody application"或"Reptin antibody characterization"在Web of Science/PubMed中筛选实验方法学明确的文献。
**Background of Reptin/RUVBL2 Antibody**
Reptin, also known as RUVBL2 (RuvB-like AAA ATPase 2), is a conserved eukaryotic protein belonging to the AAA+ (ATPases Associated with diverse cellular Activities) superfamily. It forms a heterohexameric complex with its paralog RUVBL1. functioning as an ATP-dependent molecular chaperone involved in chromatin remodeling, transcriptional regulation, DNA repair, and cellular signaling. RUVBL2 interacts with diverse protein complexes, including INO80/SWR1 chromatin remodelers, RNA polymerase II, and the TIP60 histone acetyltransferase complex, playing critical roles in cell proliferation, differentiation, and stress responses.
Antibodies targeting Reptin/RUVBL2 are essential tools for studying its expression, localization, and interactions. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in investigations of its mechanistic roles in diseases. Dysregulation of RUVBL2 is linked to cancer progression, neurodegenerative disorders, and immune dysfunctions, making it a potential therapeutic target. These antibodies help validate protein knockdown/overexpression models and assess clinical correlations in tissue samples. Researchers also use them to explore RUVBL2’s ATPase activity, structural dynamics, and partnerships in multi-protein assemblies, advancing understanding of its contributions to genome stability and cellular homeostasis.
×
