| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Il1rak; IRAK; Irak1; IRAK1-S; mPLK; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk;;IRAK1 |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human IRAK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于IRAK抗体的参考文献示例(注：文献信息为示例性质，非真实存在)：
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1. **文献名称**: *Structural basis of IRAK4 activation by phosphorylation and dimerization*
**作者**: Chen L, et al.
**摘要**: 该研究通过X射线晶体学解析IRAK4激酶结构，利用特异性抗体验证其磷酸化位点及二聚化机制，揭示了IRAK4在TLR信号中的构象变化。
2. **文献名称**: *IRAK-M ablation promotes anti-tumor immunity by modulating myeloid-derived suppressor cells*
**作者**: Smith J, et al.
**摘要**: 研究通过IRAK-M敲除小鼠模型及抗体验证，发现IRAK-M缺失可减少肿瘤微环境中免疫抑制性细胞，增强T细胞抗肿瘤活性，提示其作为癌症治疗靶点。
3. **文献名称**: *Ubiquitination of IRAK1 regulates NF-κB signaling in inflammatory responses*
**作者**: Wang Y, et al.
**摘要**: 该文利用IRAK1泛素化特异性抗体，阐明其K63泛素化修饰在炎症反应中调控NF-κB通路的关键作用，为自身免疫疾病提供新机制。
4. **文献名称**: *Development of a selective IRAK4 inhibitor using conformational-specific antibodies*
**作者**: Patel R, et al.
**摘要**: 研究通过构象特异性IRAK4抗体筛选小分子抑制剂，验证其在类风湿性关节炎模型中的疗效，为靶向IRAK4的药物开发提供策略。
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**说明**：以上文献为模拟示例，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实文献。IRAK抗体常用于研究其表达、修饰及功能，涉及免疫、癌症和药物开发等领域。
**Background of IRAK Antibodies**
Interleukin-1 receptor-associated kinase (IRAK) antibodies are essential tools for studying the IRAK family of proteins, which play pivotal roles in innate immune signaling. The IRAK family includes four members: IRAK1. IRAK2. IRAK3 (IRAK-M), and IRAK4. These serine/threonine kinases are critical mediators downstream of Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). Upon receptor activation, IRAKs form signaling complexes with adaptor proteins like MyD88. triggering downstream pathways such as NF-κB and MAPK, which drive inflammatory cytokine production.
IRAK4. the most studied member, is unique for its active kinase role in initiating signaling. IRAK1 and IRAK2 amplify signals, while IRAK-M acts as a negative regulator to prevent excessive inflammation. Dysregulation of IRAKs is linked to autoimmune diseases, chronic inflammation, and cancer.
IRAK antibodies are widely used to detect protein expression, phosphorylation status, and interactions in immune cells. They aid in elucidating IRAK-dependent mechanisms in diseases and evaluating therapeutic strategies. For instance, IRAK4 inhibitors are under investigation for treating rheumatoid arthritis, lupus, and certain cancers. However, functional redundancy among IRAK isoforms and their complex regulation pose challenges in drug development.
Overall, IRAK antibodies are indispensable for advancing research on innate immunity and inflammation, bridging molecular insights to potential clinical applications.
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