| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SORBS3 |
| Uniprot No | O60504-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADGGSP FLGRRDFVYP SSTRDPSASN GGGSPARREE KKRKAARLKF DFQAQSPKEL TLQKGDIVYI HKEVDKNWLE GEHHGRLGIF PANYVEVLPA DEIPKPIKPP TYQVLEYGEA VAQYTFKGDL EVELSFRKGE HICLIRKVNE NWYEGRITGT GRQGIFPASY VQVSREPRLR LCDDGPQLPT SPRLTAAARS ARHPSSPSAL RSPADPIDLG GQTSPRRTGF SFPTQEPRPQ TQNLGTPGPA LSHSRGPSHP LDLGTSSPNT SQIHWTPYRA MYQYRPQNED ELELREGDRV DVMQQCDDGW FVGVSRRTQK FGTFPGNYVA PV |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SORBS3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Expression and characterization of recombinant SORBS3 in mammalian cells for functional studies"*
**作者**：Tanaka K, et al.
**摘要**：研究通过哺乳动物表达系统成功表达并纯化SORBS3重组蛋白，验证其与细胞骨架蛋白(如vinculin)的相互作用，揭示其在细胞黏附中的调控作用。
2. **文献名称**：*"SORBS3 regulates mitochondrial oxidative metabolism in skeletal muscle by interacting with PGC-1α"*
**作者**：Liu Y, et al.
**摘要**：利用重组SORBS3蛋白进行体外结合实验，证明其通过与PGC-1α直接结合调控线粒体生物合成，影响骨骼肌能量代谢。
3. **文献名称**：*"Recombinant SORBS3 suppresses TGF-β signaling and attenuates fibrosis in hepatic stellate cells"*
**作者**：Wang H, et al.
**摘要**：通过体外递送重组SORBS3蛋白，发现其抑制TGF-β/Smad通路活性，减少肝星状细胞纤维化标志物表达，提示其抗纤维化潜力。
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注：上述文献为示例，实际引用需根据具体研究内容检索PubMed或Web of Science等数据库获取。
**Background of SORBS3 Recombinant Protein**
SORBS3 (sorbin and SH3 domain-containing protein 3), also known as Vinexin, is a multidomain adaptor protein encoded by the *SORBS3* gene. It belongs to the SORBS family of proteins, characterized by conserved sorbin homology (SoHo) and multiple Src homology 3 (SH3) domains. These structural features enable SORBS3 to interact with various signaling molecules, cytoskeletal components, and membrane receptors, facilitating its role in cell adhesion, signal transduction, and cytoskeletal organization.
Functionally, SORBS3 is implicated in regulating cellular processes such as migration, proliferation, and differentiation. It interacts with integrins, growth factor receptors (e.g., EGFR), and signaling proteins like c-Abl and PTP1B, influencing pathways such as PI3K/AKT and MAPK. Its involvement in cell-matrix adhesion underscores its importance in tissue homeostasis and wound healing.
Recombinant SORBS3 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and roles in diseases. Dysregulation of SORBS3 has been linked to cancer metastasis, insulin resistance, and cardiovascular disorders, making it a potential therapeutic target. Research utilizing recombinant SORBS3 aids in elucidating its molecular mechanisms and developing strategies to modulate its activity in pathological conditions.
Overall, SORBS3 recombinant protein serves as a critical tool for understanding cell signaling networks and advancing biomedical research.
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