| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 100 kDa NT receptor; Glycoprotein 95; Gp95; LDLCQ6; Neurotensin receptor 3; NT3; NTR3; SORT 1; Sortilin 1;;Sortilin |
| WB Predicted band size | 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Sortilin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Sortilin抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors*
**作者**：Jia L, et al.
**摘要**：该研究利用Sortilin特异性抗体结合免疫印迹和免疫荧光技术，揭示了Sortilin与酪氨酸激酶受体协同调控外泌体分泌的机制，为神经退行性疾病的治疗提供了新方向。
2. **文献名称**：*Progranulin binds to Sortilin to regulate lysosomal function and lipid metabolism*
**作者**：Zheng Y, et al.
**摘要**：通过Sortilin抗体的免疫共沉淀实验，作者发现Progranulin依赖Sortilin调控溶酶体功能，并证实其在脂代谢异常相关疾病(如动脉粥样硬化)中的关键作用。
3. **文献名称**：*Sortilin antibody inhibits pathological angiogenesis in murine models*
**作者**：Smith CE, et al.
**摘要**：该研究开发了一种高特异性Sortilin中和抗体，证明其能通过阻断VEGF信号通路抑制小鼠模型中病理性血管生成，为眼底病变和肿瘤治疗提供了潜在策略。
(注：以上文献信息为示例，实际引用时建议通过PubMed/Google Scholar核对准确性。)
**Background of Sortilin Antibodies**
Sortilin, a member of the vacuolar protein sorting 10 protein (VPS10P) domain receptor family, plays critical roles in intracellular protein trafficking, apoptosis, and receptor signaling. It is involved in neurotrophic signaling, lipid metabolism, and the pathogenesis of neurodegenerative disorders (e.g., Alzheimer’s disease), cardiovascular diseases, and cancer. Sortilin antibodies are essential tools for studying its expression, localization, and functional interactions.
These antibodies target specific epitopes on sortilin, enabling detection via techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry. They are widely used to investigate sortilin's role in regulating neurotrophin signaling (e.g., proNGF and proBDNF), its interaction with ligands such as progranulin and apolipoprotein E, and its involvement in disease mechanisms. For example, sortilin antibodies have helped identify its contribution to amyloid-β metabolism in Alzheimer’s models and lipid uptake in atherosclerosis.
Commercial sortilin antibodies are typically raised against peptide sequences from the intracellular or extracellular domains. Validation includes testing in knockout cell lines or tissues to confirm specificity. Researchers must select antibodies validated for their specific applications, as cross-reactivity with related VPS10P receptors (e.g., SorLA or SorCS) can occur. Recent studies also utilize sortilin antibodies in therapeutic research, such as targeting sortilin in cancer drug resistance.
Overall, sortilin antibodies are pivotal for unraveling the protein's multifaceted roles in health and disease.
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