| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APG12-like; APG12L; ATG12; Autophagy 12; Autophagy-related protein 12; FBR93; HAPG12; Ubiquitin-like protein ATG12;;ATG12 |
| WB Predicted band size | Calculated MW: 15 kDa ; Observed MW: 22,55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ATG12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Atg12(原称Apg12)抗体相关的文献摘要：
1. **文献名称**：*The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy*
**作者**：Mizushima N., et al.
**摘要**：该研究利用Atg12抗体验证了Atg12-Atg5复合物在自噬体形成中的关键作用，发现其通过类似E3泛素连接酶的机制促进LC3脂化，推动自噬膜延伸。
2. **文献名称**：*Loss of autophagy in the central nervous system causes neurodegeneration in mice*
**作者**：Hara T., et al.
**摘要**：通过Atg12抗体检测神经元特异性Atg5/Atg12缺失的小鼠模型，证明自噬缺陷导致异常蛋白积累和神经退行性病变，揭示了自噬在神经保护中的必要性。
3. **文献名称**：*Autophagy suppresses tumorigenesis through elimination of p62*
**作者**：Mathew R., et al.
**摘要**：研究利用Atg12抗体分析自噬缺陷肿瘤模型，发现Atg12缺失导致p62蛋白堆积，激活氧化应激通路并促进肿瘤发生，强调了自噬通过清除p62抑制癌症的机制。
以上文献均通过Atg12抗体在Western blot或免疫沉淀实验中验证蛋白表达或相互作用，支持自噬机制与疾病关联的研究。
The Atg12 (Autophagy-related protein 12) antibody is a crucial tool for studying the molecular mechanisms of autophagy, a conserved cellular process responsible for degrading damaged organelles, misfolded proteins, and pathogens. Atg12 is a key component of the ubiquitin-like conjugation system essential for autophagosome formation. During autophagy, Atg12 covalently binds to Atg5 via an E1/E2-like enzymatic cascade involving Atg7 and Atg10. forming the Atg12-Atg5 conjugate. This complex further interacts with Atg16L1 to promote autophagosome membrane elongation and LC3 lipidation, a critical step in autophagosome maturation.
The Atg12 antibody is widely used to detect endogenous Atg12 protein levels or its conjugated form (Atg12-Atg5) in various experimental models, including cell lines, tissues, and *in vivo* systems. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore autophagy regulation under physiological or stress conditions (e.g., nutrient deprivation, oxidative stress, or infection). It also aids in studying autophagy-related diseases, such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and infectious diseases, where autophagy dysregulation is implicated.
Validated for specificity and sensitivity, the Atg12 antibody helps dissect autophagy pathways and evaluate potential therapeutic interventions targeting this process. Its application has significantly advanced our understanding of autophagy’s role in cellular homeostasis, disease progression, and treatment responses.
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