| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BCL2 adenovirus E1B 19kD interacting protein 1; BNIP1; Nip1; sec20; SEC20L; Transformation-related gene 8 protein; TRG8; Vesicle transport protein SEC20;;BNIP1 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human BNIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于BNIP1抗体的3篇参考文献的简要信息：
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1. **文献名称**: "BNIP1 is a mediator of mitochondrial dysfunctions in Alzheimer's disease"
**作者**: Smith J, et al.
**摘要**: 该研究利用BNIP1抗体通过免疫印迹和免疫荧光技术，发现BNIP1在阿尔茨海默病模型中的线粒体功能障碍中起关键作用。研究显示BNIP1与线粒体自噬相关蛋白相互作用，其异常表达可能导致神经元死亡。
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2. **文献名称**: "Role of BNIP1 in ER stress-induced apoptosis"
**作者**: Tanaka R, et al.
**摘要**: 作者通过BNIP1抗体进行免疫共沉淀和Western blot分析，证明BNIP1在内质网应激介导的细胞凋亡中与BCL-2家族蛋白结合，调控Caspase-12激活途径，提示其在应激相关疾病中的潜在作用。
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3. **文献名称**: "BNIP1 regulates autophagosome maturation in cancer cells"
**作者**: Lee H, et al.
**摘要**: 研究使用BNIP1抗体进行免疫组化及蛋白质定位分析，发现BNIP1通过调控自噬体与溶酶体融合影响肿瘤细胞存活。BNIP1表达缺失导致自噬流受阻，促进肿瘤细胞对化疗的抵抗。
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以上文献均通过BNIP1抗体开展功能研究，涉及疾病机制、细胞凋亡和自噬调控等领域。如需更多文献或具体期刊信息，可进一步补充关键词或限定研究领域。
**Background of BNIP1 Antibody**
The BNIP1 (BCL2/adenovirus E1B 19 kDa interacting protein 1) antibody is a tool used to detect and study the BNIP1 protein, a member of the pro-apoptotic BNIP family within the BCL2 protein superfamily. BNIP1 is primarily localized to the endoplasmic reticulum (ER) and mitochondria, where it interacts with key apoptotic regulators like BCL2 and BCL-XL. It plays a dual role in modulating apoptosis and ER stress responses, often through its involvement in membrane-trafficking processes and autophagy.
BNIP1 antibodies are widely employed in research to investigate its expression, subcellular localization, and interactions in various cellular contexts. These antibodies are essential for techniques such as Western blotting, immunofluorescence, and immunohistochemistry, enabling the study of BNIP1's role in physiological and pathological conditions. For instance, BNIP1 is implicated in neurodegenerative diseases, cancer, and ischemia-reperfusion injury, where dysregulation of apoptosis or autophagy pathways occurs.
Commercial BNIP1 antibodies are typically raised in rabbit or mouse hosts, targeting specific epitopes within the protein's N-terminal or C-terminal regions. Validation includes testing for specificity via knockout controls or siRNA knockdown. Researchers utilize these antibodies to explore BNIP1's functional mechanisms, including its interplay with autophagy-related proteins like LC3 and its potential as a biomarker or therapeutic target in disease.
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