| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX5 |
| Uniprot No | Q9Y5X3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404aa |
| 氨基酸序列 | MASMTGGQQMGRGEFMAAVPELLQQQEEDRSKLRSVSVDLNVDPSLQIDI PDALSERDKVKFTVHTKTTLPTFQSPEFSVTRQHEDFVWLHDTLIETTDY AGLIIPPAPTKPDFDGPREKMQKLGEGEGSMTKEEFAKMKQELEAEYLAV FKKTVSSHEVFLQRLSSHPVLSKDRNFHVFLEYDQDLSVRRKNTKEMFGG FFKSVVKSADEVLFTGVKEVDDFFEQEKNFLINYYNRIKDSCVKADKMTR SHKNVADDYIHTAACLHSLALEEPTVIKKYLLKVAELFEKLRKVEGRVSS DEDLKLTELLRYYMLNIEAAKDLLYRRTKALIDYENSNKALDKARLKSKD VKLAEAHQQECCQKFEQLSESAKEELINFKRKRVAAFRKNLIEMSELEIK HARNNVSLLQSCIDLFKNN |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX5重组蛋白的3篇参考文献示例(注：以下内容为虚构，仅用于示例)：
1. **文献名称**：*Structural and functional analysis of SNX5 reveals its role in membrane remodeling*
**作者**：Zhang L, et al.
**摘要**：本研究通过重组表达人源SNX5蛋白，解析了其与SNX6形成的异二聚体结构，并证明其通过BAR结构域介导膜变形，参与内体分选过程。
2. **文献名称**：*Recombinant SNX5 facilitates HCV entry by interacting with viral glycoproteins*
**作者**：Wang Y, et al.
**摘要**：利用大肠杆菌系统表达并纯化SNX5重组蛋白，发现其通过结合丙型肝炎病毒(HCV)的E2糖蛋白，调控病毒内吞途径，为抗病毒靶点提供依据。
3. **文献名称**：*SNX5 regulates autophagy via lysosomal targeting in a phosphorylation-dependent manner*
**作者**：Kim S, et al.
**摘要**：通过重组SNX5蛋白的体外磷酸化实验，揭示了其激酶依赖性磷酸化修饰如何影响与自噬相关蛋白LC3的互作，进而调控溶酶体功能。
(注：以上文献为模拟示例，实际引用请查询具体数据库。)
SNX5 (Sorting Nexin 5) is a member of the sorting nexin family, a group of proteins characterized by the presence of a phox homology (PX) domain that facilitates interactions with phosphoinositide-enriched membranes. As a recombinant protein, SNX5 is engineered for in vitro studies to elucidate its role in intracellular membrane trafficking, organelle dynamics, and cellular signaling. It functions primarily within the endosomal system, where it collaborates with other sorting nexins (e.g., SNX1 or SNX6) to form heterodimers critical for cargo sorting and membrane deformation. SNX5 is a component of the retromer complex, which mediates the retrieval of transmembrane proteins from endosomes to the trans-Golgi network, ensuring proper recycling of receptors like the cation-independent mannose-6-phosphate receptor.
Structurally, recombinant SNX5 often retains its PX domain and a C-terminal BAR (Bin/Amphiphysin/Rvs) domain, enabling membrane curvature sensing and scaffolding. Dysregulation of SNX5 has been linked to metabolic disorders, neurodegenerative diseases, and cancer, underscoring its importance in maintaining cellular homeostasis. For instance, impaired SNX5 function disrupts insulin receptor trafficking, contributing to insulin resistance, while its interaction with pathogenic proteins (e.g., amyloid precursor protein) may influence Alzheimer’s disease progression.
Recombinant SNX5 is widely used to study endosome-to-Golgi transport mechanisms, screen therapeutic compounds targeting membrane trafficking pathways, and analyze protein-protein interaction networks via assays like pull-downs or fluorescence microscopy. Its production in bacterial or mammalian expression systems ensures high purity and activity for functional studies, making it a vital tool in cell biology and drug discovery research.
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