| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX3 |
| Uniprot No | O60493 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-162aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAETVADTRRLITKPQNLNDAYGPPSNFLE IDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSE LERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVA GHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX3重组蛋白的3篇参考文献示例(文献信息为虚构，仅供示例参考)：
1. **文献名称**：*Structural insights into SNX3-mediated cargo recognition in retromer-dependent trafficking*
**作者**：Harrison, M.S. et al.
**摘要**：本研究解析了SNX3重组蛋白的晶体结构，揭示了其通过PX结构域特异性结合磷脂酰肌醇3-磷酸(PI3P)的分子机制，并证明SNX3与Retromer复合体协作调控溶酶体酶运输的关键作用。
2. **文献名称**：*SNX3 regulates endosomal membrane remodeling through its BAR domain*
**作者**：Chen, L. et al.
**摘要**：通过体外重组SNX3蛋白实验，发现其BAR结构域可诱导脂膜形成管状结构，表明SNX3在细胞内吞运输过程中通过膜变形参与货物分选，并依赖其寡聚化状态发挥功能。
3. **文献名称**：*Functional analysis of SNX3 in Wnt signaling via β-catenin recycling*
**作者**：Xu, Y. et al.
**摘要**：利用重组SNX3蛋白进行互作实验，发现其直接结合β-连环蛋白(β-catenin)，调控Wnt信号通路的活性，揭示了SNX3在结肠癌细胞中影响肿瘤发生的新机制。
4. **文献名称**：*SNX3 deficiency disrupts iron homeostasis via impaired transferrin receptor recycling*
**作者**：Wang, Q. et al.
**摘要**：通过基因敲除模型和SNX3重组蛋白回补实验，证明SNX3通过介导转铁蛋白受体(TfR)的再循环维持细胞铁稳态，其功能缺失导致铁代谢紊乱相关疾病。
(注：以上文献为示例性内容，实际研究需参考真实发表的论文。)
SNX3 (Sorting Nexin 3) is a member of the sorting nexin family, a group of proteins characterized by their phosphoinositide-binding Phox homology (PX) domains. These proteins play critical roles in membrane trafficking, cellular signaling, and organelle dynamics. SNX3. specifically, is a small monomeric protein (~20 kDa) that lacks the additional structural domains (e.g., BAR or SH3) found in other sorting nexins, making it unique in its functional simplicity. It localizes primarily to early endosomes and regulates retrograde transport of cargo from endosomes to the trans-Golgi network (TGN), a process essential for maintaining cellular homeostasis.
SNX3 interacts with phosphatidylinositol-3-phosphate (PI3P) via its PX domain, anchoring it to endosomal membranes. It facilitates cargo recognition and sorting by coupling with retromer complexes, which mediate the retrieval of specific receptors (e.g., Wntless for Wnt protein secretion or the iron transporter DMT1). Studies highlight its role in Wnt/β-catenin signaling, iron metabolism, and neuronal development. Dysregulation of SNX3 has been linked to neurodegenerative diseases, cancer progression, and iron-deficiency disorders.
Recombinant SNX3 protein is engineered for in vitro studies to dissect its molecular interactions, structural dynamics, and functional roles. Produced using bacterial or mammalian expression systems, it often includes tags (e.g., His-tag) for purification. Research applications include binding assays with lipid vesicles, co-immunoprecipitation with retromer subunits, and screening for small-molecule modulators. Its recombinant form has advanced understanding of endosomal trafficking mechanisms and potential therapeutic targeting of SNX3-associated pathologies.
Overall, SNX3 exemplifies how a compact, domain-specific protein can orchestrate complex cellular processes, with recombinant tools enabling deeper exploration of its biological and clinical significance.
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