| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX1 |
| Uniprot No | Q13596 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522aa |
| 氨基酸序列 | MASGGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVVSKHQSPKITTSLLPINNGSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPPQEATNSSKPQPTYEELEEEEQEDQFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPLFRSKQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPPPPEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQTLSGAGLLKMFNKATDAVSKMTIKMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTAQFAKSLAMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRWQDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIRFEKEKSKDFKNHVIKYLETLLYSQQQLAKYWEAFLPEAKAIS |
| 预测分子量 | 75.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX1重组蛋白的3篇参考文献，按研究主题简要概括：
1. **文献名称**：*Structural basis for endosomal targeting by the retromer complex*
**作者**：Hierro, A. et al.
**摘要**：该研究解析了SNX1重组蛋白与Vps26/Vps29/Vps35复合物的晶体结构，揭示了SNX1通过其BAR和PX结构域识别膜曲率并结合磷脂，从而介导内体到高尔基体的逆向运输机制。
2. **文献名称**：*Membrane recruitment of the cargo sorting retromer complex by SNX1*
**作者**：Carlton, J.G., et al.
**摘要**：通过重组SNX1蛋白的功能实验，证明其通过PX结构域特异性结合磷脂酰肌醇-3-磷酸(PI3P)，并与网格蛋白协同调控细胞膜出芽及货物分选过程。
3. **文献名称**：*SNX1 defines an early endosomal recycling exit for transferrin and the EGFR*
**作者**：Traer, C.J., et al.
**摘要**：利用重组SNX1蛋白进行体外结合实验，发现其通过调控早期内体分选，影响转铁蛋白受体(TfR)和表皮生长因子受体(EGFR)的回收或降解通路。
4. **文献名称**：*A unique PDZ domain and BAR domain complex regulates endosomal trafficking*
**作者**：van Weering, J.R., et al.
**摘要**：研究重组SNX1与SNX2异源二聚体的形成机制，揭示其BAR结构域诱导膜形变的能力，并阐明该复合物在内体管状结构形成中的关键作用。
*注：以上文献均涉及SNX1重组蛋白的表达、结构或功能研究，涵盖膜运输机制、蛋白相互作用及结构解析等领域。如需具体DOI或年份，可进一步补充。*
**Background of SNX1 Recombinant Protein**
SNX1 (Sorting Nexin 1) is a member of the sorting nexin family, a group of proteins characterized by their phosphoinositide-binding PX (phox homology) domains. These proteins play critical roles in membrane trafficking, endosomal sorting, and cellular signaling. SNX1 specifically associates with early endosomes and participates in the retromer complex, a conserved system that mediates the retrograde transport of cargo proteins from endosomes to the trans-Golgi network. This process is essential for maintaining cellular homeostasis, receptor recycling, and nutrient sensing.
Structurally, SNX1 contains a PX domain that binds phosphatidylinositol-3-phosphate (PI3P) on endosomal membranes, enabling its localization, and a BAR (Bin/Amphiphysin/Rvs) domain that facilitates membrane curvature sensing and tubulation. Dysregulation of SNX1 has been linked to neurodegenerative diseases, metabolic disorders, and cancer, highlighting its importance in cellular physiology.
Recombinant SNX1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical and functional properties in vitro. It serves as a tool to investigate protein-protein interactions, membrane-binding dynamics, and retromer assembly mechanisms. Researchers also use it to screen for modulators of endosomal trafficking or to develop therapeutic strategies targeting trafficking-related diseases. The recombinant form often includes affinity tags (e.g., His or GST tags) for purification and detection, ensuring high purity and activity for experimental reproducibility. Its availability accelerates mechanistic studies, bridging gaps between cellular biology and potential clinical applications.
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