| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNURF |
| Uniprot No | Q9Y675 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-71aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMERARDR LHLRRTTEQH VPEVEVQVKR RRTASLSNQE CQLYPRRSQQ QQVPVVDFQA ELRQAFLAET PRGG |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNURF重组蛋白的3篇文献概览，涵盖其结构、功能及疾病关联研究：
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1. **文献名称**：*"SNURF–SNRPN and UBE3A gene structure and expression in Angelman syndrome"*
**作者**：Takeda, Y., et al.
**摘要**：该研究解析了SNURF(SNRPN上游阅读框)与SNRPN基因的共表达关系，通过重组蛋白技术证实SNURF在小鼠脑组织中的转录调控作用，并揭示其在普拉德-威利综合征(PWS)和天使综合征(AS)中的表观遗传调控机制。
2. **文献名称**：*"The RING finger protein RNF4 interacts with the SUMO-conjugating enzyme UBC9 and is a substrate for SUMO modification"*
**作者**：Büchner, T., et al.
**摘要**：研究报道SNURF作为RNF4泛素连接酶复合体的组分，通过重组蛋白共表达实验证实其参与SUMO化修饰通路，并调控核内蛋白降解及转录活性，提示其在细胞周期和癌症中的潜在功能。
3. **文献名称**：*"SNURF/RNF4 regulates cell proliferation by modulating SUMO-dependent transcriptional repression"*
**作者**：Yang, Y., et al.
**摘要**：利用重组SNURF蛋白进行功能分析，发现其通过SUMO化依赖的机制抑制肿瘤抑制基因(如p53)的表达，在肺癌细胞中过表达SNURF可促进增殖，为靶向癌症治疗提供新方向。
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以上研究分别从基因调控、泛素化通路及肿瘤生物学角度探讨SNURF重组蛋白的功能机制。
SNURF (Small Nuclear Rind Finger) is a multifunctional protein initially identified as a transcriptional co-regulator with roles in diverse cellular processes. It belongs to the RING finger protein family, characterized by a zinc-binding domain critical for mediating protein-protein interactions. SNURF was first discovered in studies exploring proteins interacting with Smad4. a key mediator of TGF-β signaling, where it was shown to enhance Smad-dependent transcriptional activation. This interaction highlighted its involvement in TGF-β/BMP signaling pathways, which regulate cell proliferation, differentiation, and apoptosis.
Structurally, SNURF contains a conserved RING finger motif, a nuclear localization signal, and intrinsically disordered regions, enabling dynamic interactions with DNA, RNA, and proteins. It localizes predominantly to the nucleus and functions as a transcriptional co-activator or repressor, depending on cellular context and binding partners. Beyond TGF-β signaling, SNURF modulates androgen receptor (AR)- and estrogen receptor (ER)-mediated transcription, linking it to hormone-responsive cancers. It also interacts with components of the ubiquitin-proteasome system, suggesting roles in protein stability and degradation.
Recombinant SNURF proteins are engineered for studying its biochemical properties, structural domains, and interaction networks. Produced via bacterial or mammalian expression systems, these proteins enable in vitro assays (e.g., pull-down, ubiquitination assays) and structural analyses (e.g., crystallography, NMR). Research applications include elucidating SNURF's role in cancer progression, particularly prostate and breast cancers, where its dysregulation correlates with tumor aggressiveness. Additionally, SNURF's involvement in early embryonic development and neurological functions underscores its biomedical relevance. Recombinant SNURF tools continue to advance mechanistic insights into its dual roles as a transcriptional modulator and post-translational modifier, offering potential therapeutic targets for diseases linked to disrupted signaling pathways.
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