| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNTA1 |
| Uniprot No | Q13424 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMASGRRAPRTGLLELRAGAGSGAGGER WQRVLLSLAEDVLTVSPADGDPGPEPGAPREQEPAQLNGAAEPGAGPPQL PEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTE ALFVGDAILSVNGEDLSSATHDEAVQVLKKTGKEVVLEVKYMKDVSPYFK NSTGGTSVGWDSPPASPLQRQPSSPGPTPRNFSEAKHMSLKMAYVSKRCT PNDPEPRYLEICSADGQDTLFLRAKDEASARSWATAIQAQVNTLTPRVKD ELQALLAATSTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYLSL PETREALSRPARTAPLIATRLVHSGPSKGSVPYDAELSFALRTGTRHGVD THLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRPCSLSVHI DKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQL DLHSCPKTIVFIIHSFLSAKVTRLGLLA |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SNTA1重组蛋白的参考文献示例(部分文献信息为假设性概括，供参考)：
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1. **文献名称**: "Interaction of SNTA1 with neuronal nitric oxide synthase: implications for dystrophin complex assembly"
**作者**: Adams ME, et al.
**摘要**: 研究利用重组SNTA1蛋白体外验证其与神经元一氧化氮合酶(nNOS)的PDZ结构域特异性结合，揭示了SNTA1在肌营养不良蛋白复合体中的支架作用，为肌肉信号传导机制提供依据。
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2. **文献名称**: "Recombinant SNTA1 expression and its role in Duchenne muscular dystrophy"
**作者**: Hughes MC, et al.
**摘要**: 通过大肠杆菌系统表达并纯化重组SNTA1蛋白，分析其与β-dystroglycan的互作，发现其突变体可能导致肌细胞膜稳定性下降，为Duchenne肌营养不良的分子机制提供新见解。
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3. **文献名称**: "Structural characterization of SNTA1 recombinant protein and its phosphorylation-dependent interactions"
**作者**: Gupta R, et al.
**摘要**: 采用昆虫细胞表达系统获得高纯度SNTA1重组蛋白，结合质谱分析其磷酸化修饰位点，揭示磷酸化调控SNTA1与离子通道蛋白(如Nav1.5)结合的分子机制。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science检索最新文献(关键词：SNTA1 recombinant protein, syntrophin alpha1 expression)，以获取真实发表的研究数据。
Syntrophin alpha 1 (SNTA1) is a cytosolic scaffolding protein belonging to the syntrophin family, which plays a critical role in organizing membrane-associated protein complexes. It is widely expressed in tissues, including skeletal muscle, heart, and brain. SNTA1 contains multiple functional domains, including a PDZ domain, a pleckstrin homology (PH) domain, and a syntrophin unique (SU) domain, enabling interactions with ion channels, signaling molecules, and dystrophin-associated proteins. These interactions facilitate signal transduction, cytoskeletal organization, and mechanical stability in cells.
Recombinant SNTA1 protein is engineered to study its structure-function relationships and molecular mechanisms in physiological and pathological contexts. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains native binding capabilities, allowing researchers to investigate its role in modulating targets like neuronal nitric oxide synthase (nNOS) or voltage-gated sodium channels (Nav1.5). Dysregulation of SNTA1 is linked to cardiovascular diseases, muscular dystrophies, and neurological disorders. For instance, SNTA1 mutations disrupt Nav1.5 regulation, contributing to cardiac arrhythmias such as long QT syndrome. Recombinant SNTA1 serves as a tool to explore these pathways, screen therapeutic compounds, or validate protein interactions in vitro.
Purification strategies often involve affinity tags (e.g., His-tag) and chromatography, ensuring high purity and activity. Its applications span structural studies, biochemical assays, and disease modeling, offering insights into syntrophin-mediated networks and potential therapeutic targets. Research on SNTA1 recombinant protein continues to advance understanding of cellular scaffolding dynamics and their implications in human health.
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