| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPE |
| Uniprot No | P62304 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-92aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNRPE重组蛋白的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询确认)：
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1. **文献名称**: *Recombinant Expression and Functional Analysis of the Human SNRPE Protein in Splicing Mechanisms*
**作者**: Smith J, Lee A, et al.
**摘要**: 本研究报道了人类SNRPE蛋白在大肠杆菌中的重组表达与纯化，并通过体外剪接实验验证了其在小核核糖核蛋白复合体(snRNP)组装中的作用。研究揭示了SNRPE突变对pre-mRNA剪接效率的影响。
2. **文献名称**: *Structural Insights into SNRPE's Role in snRNP Assembly via Cryo-EM*
**作者**: Zhang Y, Wang H, et al.
**摘要**: 通过冷冻电镜解析了重组SNRPE蛋白与Sm蛋白复合体的高分辨率结构，阐明了SNRPE在snRNP核心形成中的关键结合位点，为剪接体相关疾病的机制研究提供结构基础。
3. **文献名称**: *SNRPE Mutations in Genetic Disorders: Recombinant Protein-Based Functional Rescue*
**作者**: García R, Patel N, et al.
**摘要**: 本文利用HEK293细胞表达重组SNRPE蛋白，证实其在患者细胞模型中可恢复因SNRPE突变导致的剪接缺陷，提示基因治疗中补充功能性SNRPE的潜力。
4. **文献名称**: *Optimized Purification of Recombinant SNRPE for High-Throughput Drug Screening*
**作者**: Kim T, O'Connor B, et al.
**摘要**: 开发了一种高效层析法纯化重组SNRPE蛋白的流程，用于靶向snRNP的抗癌药物筛选，验证了其在抑制剂结合实验中的稳定性与活性。
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建议通过 **PubMed** 或 **Web of Science** 输入关键词“SNRPE recombinant”或“SNRPE expression”获取真实文献。如需具体文章，可进一步提供研究方向(如结构、疾病、技术方法)以便精准筛选。
SNRPE (Small Nuclear Ribonucleoprotein Polypeptide E) is a core component of the spliceosome, a dynamic molecular machinery essential for pre-mRNA splicing in eukaryotic cells. As part of the Sm protein family, SNRPE binds to uridine-rich small nuclear RNAs (snRNAs) like U1. U2. U4. and U5. forming stable ribonucleoprotein (RNP) complexes. These complexes recognize splice sites on pre-mRNA, facilitating the excision of introns and ligation of exons to produce mature mRNA. SNRPE contains a conserved Sm domain that mediates its interaction with snRNAs and other Sm proteins (e.g., SNRPB, SNRPD), ensuring proper spliceosome assembly and catalytic activity.
Recombinant SNRPE protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) for structural and functional studies. Its recombinant form enables researchers to investigate spliceosome dynamics, RNA-protein interactions, and splicing regulation mechanisms. Dysregulation of SNRPE has been implicated in diseases, including cancer and autoimmune disorders. For example, autoantibodies targeting SNRPE and other Sm proteins are biomarkers in systemic lupus erythematosus (SLE). Recombinant SNRPE also serves as an antigen in diagnostic assays to detect such autoantibodies.
In drug discovery, recombinant SNRPE aids in screening compounds that modulate splicing activity, potentially addressing splicing-related pathologies. Structural studies using recombinant protein have clarified its role in snRNA binding and spliceosome architecture. Despite its small size (~11 kDa), SNRPE's conserved function across eukaryotes underscores its evolutionary importance in gene expression regulation. Ongoing research focuses on its post-translational modifications, disease associations, and therapeutic targeting, highlighting its relevance in molecular biology and biomedicine.
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