Rat Interleukin 1β,IL-1β ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08055r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠白介素1β (IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的IL1B含量。IL1B即白介素-1β，是一种重要的促炎细胞因子。在炎症反应背景下，它参与免疫调节过程。研究机制上，IL1B可激活下游信号通路，介导炎症细胞的募集与活化，进而放大炎症反应，与多种炎症相关疾病的发生发展密切相关。试剂盒检测范围为0.156 ng/ml-10 ng/ml.，灵敏度为0.039 ng/ml.。适用于实验室炎症模型构建、免疫调控机制研究、药物抗炎效果评估等科研场景，通过定量分析不同处理条件下IL-1β的动态变化，为炎症相关疾病的基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
缩写：
IL1B
Uniprot No.：
Q63264
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
0.156 ng/ml-10 ng/ml.
灵敏度：
0.039 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-96
1:2	Average %	92
	Range %	90-105
1:4	Average %	94
	Range %	91-103
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.903	2.834	2.868	2.689
5	2.208	2.167	2.187	2.008
2.5	1.563	1.508	1.536	1.356
1.25	0.872	0.859	0.865	0.686
0.625	0.579	0.551	0.565	0.386
0.312	0.363	0.353	0.358	0.178
0.156	0.284	0.274	0.279	0.099
0	0.184	0.175	0.180