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产品描述：兔子白介素1β (IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B即白介素-1β，是一种炎症细胞因子，在炎症反应、免疫调节等生理病理过程中扮演重要角色。研究表明，它能激活下游信号通路，介导炎性反应。异常表达与多种疾病如关节炎、心血管疾病等密切相关，是药物研发的热门靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于体外实验中对动物模型（如关节炎、脓毒症等疾病模型）的炎症机制研究、药物干预效果评估或免疫调节通路分析；为科研工作者在基础医学、药理学及免疫学领域提供灵敏稳定的检测工具，支持细胞因子网络调控的深入探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
缩写：IL1B
Uniprot No.：P14628
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 85-105 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 85-103 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 82-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 82-104 | ||||
| EDTA plasma (n=4) | 95 | 85-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.8522.7132.7832.6742502.2352.2452.2402.1311251.4241.3921.4081.29962.50.7530.7840.7690.66031.20.4470.4330.4400.33115.60.2660.2820.2740.1657.80.2020.1950.1990.09000.1110.1060.109 | ||||||
货期：3-5 working days
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