Rat Interleukin 1α ,IL-1α ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04622r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠白介素1α(IL-1α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的IL1A含量。IL1A 是一种细胞因子，在炎症和免疫反应中扮演关键角色。其背景上，它与多种疾病如自身免疫病、炎症性疾病相关。研究机制显示，IL1A 可激活下游信号通路，引发机体免疫细胞反应和炎症因子释放，是潜在的疾病治疗靶点。试剂盒检测范围为78 pg/ml-5000 pg/ml.，灵敏度为19.5 pg/ml.。适用于科研领域中对炎症机制、免疫调控模型及药物干预效果的评估，尤其适合同时分析体液样本（血清/血浆）与组织局部IL - 1α表达水平。为研究IL - 1α在先天免疫应答、细胞应激反应及病理状态下的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：
IL1A
Uniprot No.：
P04822
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
78 pg/ml-5000 pg/ml.
灵敏度：
19.5 pg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	98
	Range %	91-104
1:4	Average %	101
	Range %	98-107
1:8	Average %	89
	Range %	84-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.657	2.556	2.607	2.425
2500	2.123	2.022	2.073	1.891
1250	1.468	1.447	1.458	1.276
625	0.927	0.916	0.922	0.740
312	0.568	0.579	0.574	0.392
156	0.415	0.404	0.410	0.228
78	0.312	0.300	0.306	0.124
0	0.180	0.183	0.182