| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ctcf; CTCFL paralog; MRD21;;CTCF |
| WB Predicted band size | Calculated MW: 83 kDa ; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CTCF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于CTCF抗体的参考文献，按文献标题、作者和摘要内容概括整理：
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1. **标题**: "CTCF: An Architectural Protein Bridging Genome Structure and Function"
**作者**: Phillips-Cremins, J.E. & Corces, V.G.
**摘要**: 该综述总结了CTCF作为染色质结构组织核心蛋白的功能，重点讨论了其通过介导染色质环化和绝缘子元件调控基因表达的作用。文中强调了高质量CTCF抗体在染色质构象捕获(如Hi-C和ChIP-seq)中的重要性，并比较了不同抗体在检测CTCF结合位点时的特异性和灵敏度。
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2. **标题**: "Cohesin and CTCF Differentially Regulate Chromatin Architecture"
**作者**: Rao, S.S.P. et al.
**摘要**: 本研究通过高分辨率Hi-C技术结合CTCF抗体(Abcam, ab70303)的ChIP-seq分析，揭示了CTCF与黏连蛋白(cohesin)在染色质区室化和拓扑相关域(TADs)形成中的协同与竞争关系。实验验证了CTCF抗体在检测边界元件中的可靠性，并证明其结合位点的动态变化影响三维基因组结构。
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3. **标题**: "Comprehensive Comparison of Commercial Anti-CTCF Antibodies for Chromatin Immunoprecipitation"
**作者**: Wang, H. et al.
**摘要**: 研究系统评估了五种市售CTCF抗体(包括Millipore 07-729、Santa Cruz sc-15914等)在ChIP实验中的性能。通过对比不同细胞系中的结合位点覆盖度、信噪比和重复性，发现某些抗体在特定条件下(如交联时间、染色质片段化程度)表现更优，为实验者选择抗体提供了数据支持。
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4. **标题**: "CTCF Binding Site Sequence Differences Are Associated with Unique Regulatory and Functional Traits"
**作者**: Ong, C.T. & Corces, V.G.
**摘要**: 该研究利用CTCF特异性抗体(Active Motif 61311)进行ChIP-seq，结合突变分析，揭示了CTCF结合位点的序列变异如何影响其绝缘子活性及与不同蛋白(如RNA聚合酶II)的相互作用。实验证实了抗体在区分CTCF不同功能状态(如磷酸化修饰)中的应用潜力。
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**备注**：以上文献为示例性质，具体引用时请以实际发表的论文信息为准。部分文献可能需要通过PubMed或期刊官网检索完整标题及作者。
CTCF (CCCTC-binding factor) is a highly conserved zinc finger protein critical for organizing 3D chromatin architecture and regulating gene expression. It mediates chromatin looping, insulates transcriptional domains, and establishes boundaries between topologically associating domains (TADs). As a key architectural protein, CTCF influences genomic imprinting, X-chromosome inactivation, and enhancer-promoter interactions.
CTCF-specific antibodies are essential tools for studying these mechanisms through techniques like chromatin immunoprecipitation (ChIP-seq), immunofluorescence (IF), and Western blotting (WB). They enable genome-wide mapping of CTCF binding sites, revealing its role in maintaining chromatin structure and epigenetic regulation. Commercial CTCF antibodies are typically raised against epitopes in the N-terminal or C-terminal regions, with validation in specific applications. However, variability exists between clones (e.g., Millipore 07-729 vs. Active Motif 61292) in specificity and cross-reactivity, requiring careful experimental optimization.
Research using CTCF antibodies has advanced understanding of diseases linked to chromatin dysregulation, including cancers and developmental disorders. Despite challenges in batch consistency and epitope accessibility, these antibodies remain indispensable for exploring genome organization and transcriptional control mechanisms.
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