首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素17F(IL17F)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17F含量。IL17F是重要靶点。它属IL-17细胞因子家族，可调节免疫反应。研究发现，其参与多种炎症和自身免疫疾病的发病机制，过度表达会引发异常免疫。目前围绕它开发拮抗剂等药物，以阻断其信号传导治疗相关疾病。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于科研领域中对大鼠模型中IL - 17F表达水平的动态监测，如炎症性疾病机制研究、免疫调节实验或药物干预效果评估等场景，为探索IL - 17F在免疫系统中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il17fInterleukin-17F ELISA Kit; IL-17F ELISA Kit
缩写：IL17F
Uniprot No.：Q5BJ95
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:2 | Average % | 83 | |||||
| Range % | 80-86 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 80-90 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of rat IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-90 | |||||
| EDTA plasma (n=4) | 104 | 100-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.8062.7692.7882.6935002.3042.4012.3532.2582501.5351.6051.5701.4751250.6800.6980.6890.59462.50.3430.3510.3470.25231.20.2020.1990.2010.10615.60.1270.1290.1280.03300.0950.0940.095 | |||||||
货期：3-5 working days
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