| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T box brain 1; T-brain-1; TBR 1; Tbr1; TES 56;;TBR1 |
| WB Predicted band size | 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TBR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TBR1抗体的3篇参考文献及其摘要概括：
1. **《T-brain-1: A homolog of Brachyury whose expression defines molecularly distinct domains within the cerebral cortex》**
- **作者**: Bulfone, A. 等(1995)
- **摘要**: 该研究首次报道了TBR1作为大脑皮质发育的关键转录因子。通过免疫组化技术使用TBR1抗体，发现其特异性地标记了端脑中的深层皮质神经元(如第VI层)，并揭示了其在小鼠胚胎期皮质分层中的关键调控作用，为后续研究皮质神经元亚型分化奠定了基础。
2. **《Tbr1 regulates differentiation of the preplate and layer 6》**
- **作者**: Hevner, R.F. 等(2001)
- **摘要**: 研究利用TBR1抗体结合原位杂交技术，证实了TBR1在早期皮质发育中对前板(preplate)分化和第VI层神经元成熟的调控功能。实验表明，Tbr1基因敲除小鼠皮质结构异常，提示TBR1在神经元迁移和突触形成中的必要性。
3. **《Decreased TBR1 mRNA and protein expression in autism spectrum disorder》**
- **作者**: Notwell, J.H. 等(2016)
- **摘要**: 该研究通过Western blot和免疫荧光技术，分析了自闭症患者脑组织中TBR1的表达水平，发现其mRNA和蛋白表达显著降低。结合染色质分析，提出TBR1可能通过调控下游自闭症相关基因(如CNTNAP2)参与疾病发生，为神经发育障碍的机制提供了新见解。
这些文献展示了TBR1抗体在定位神经元亚群、解析皮质发育机制及疾病关联研究中的关键应用。
TBR1 (T-box brain protein 1) is a transcription factor belonging to the T-box gene family, which plays critical roles in brain development. It is particularly essential for the differentiation and laminar organization of neurons in the cerebral cortex, amygdala, and olfactory bulbs. During cortical development, TBR1 regulates the specification of glutamatergic projection neurons in deep cortical layers (e.g., layer VI) and is involved in axonal guidance and synaptic connectivity. Its expression is tightly associated with neuronal maturation, making it a key marker for studying cortical layer formation and neuronal subtype identity.
TBR1 antibodies are widely used in neuroscience research to identify and characterize specific neuronal populations, particularly in immunohistochemistry, immunofluorescence, and Western blotting. These antibodies target epitopes within the conserved T-box DNA-binding domain or other regions of the TBR1 protein, enabling visualization of its spatial and temporal expression patterns. Dysregulation of TBR1 has been linked to neurodevelopmental disorders, including autism spectrum disorders (ASD), intellectual disability, and epilepsy, often due to mutations in the *TBR1* gene. Consequently, TBR1 antibodies are valuable tools for investigating disease mechanisms, cortical malformations, and neural circuit assembly. Their specificity and reliability have made them indispensable in both developmental neurobiology and translational research aimed at understanding brain disorders.
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