首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素17(IL-17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17A含量。IL17A是一种细胞因子。其作为靶点，在自身免疫性疾病如银屑病、强直性脊柱炎等背景下备受关注。研究机制主要是它能介导炎症反应，促进促炎细胞因子释放，影响免疫细胞功能，通过阻断IL17A有望治疗这些与炎症相关的疾病。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，该产品广泛应用于炎症相关疾病动物模型研究（如关节炎、肠炎等）、免疫调控机制探索以及药物干预效果评价等领域，例如通过检测组织匀浆中的IL - 17水平分析局部炎症状态，或利用血浆样本评估系统性免疫应答。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il17a ELISA Kit; Ctla8 ELISA Kit; Il17Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit
缩写：IL17A
Uniprot No.：Q61453
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.43 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-95 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of rat IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-98 | ||||
| EDTA plasma (n=4) | 93 | 88-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 60001.8651.8021.8341.739 30001.4181.3761.3971.302 15001.0361.0041.0200.925 7500.6240.5890.6070.512 3750.3790.3640.3720.277 187.50.2110.2090.2100.115 93.750.1320.1220.1270.032 00.0990.0910.095 | ||||||
货期：3-5 working days
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