| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MYH6; MYH7; MYHC; MYHCA; MYHCB; Myosin heavy chain 6; Myosin heavy chain; Myosin-6;;Myosin heavy chain 6/7 |
| WB Predicted band size | Calculated MW: 223,224 kDa ; Observed MW: 224 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Myosin heavy chain 6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于Myosin Heavy Chain(MyHC)抗体的代表性文献，按发表时间排序：
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1. **文献名称**：*Myosin heavy chain isoforms in mammalian skeletal muscle*
**作者**：Schiaffino, S., et al.
**摘要**：该研究系统分析了哺乳动物骨骼肌中Myosin Heavy Chain(MyHC)的多种异构体，利用特异性抗体区分不同肌纤维类型(如I型、IIa、IIx、IIb)，并探讨其在肌肉功能和疾病中的表达差异。
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2. **文献名称**：*Toward a better understanding of skeletal muscle fibrosis*
**作者**：Ackermann, M., et al.
**摘要**：文章通过MyHC抗体(针对慢速和快速肌球蛋白)标记肌肉纤维，研究肌纤维化过程中的再生障碍，揭示了纤维化组织中MyHC表达异常与肌肉功能退化的关联。
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3. **文献名称**：*Cardiac myosin motor regulation by phosphorylation*
**作者**：Luther, P.K., et al.
**摘要**：研究利用MyHC抗体(特异性靶向心肌β-MyHC)探究心肌收缩的分子机制，发现磷酸化修饰对肌球蛋白马达活性的调控作用，为心脏病机制提供新见解。
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4. **文献名称**：*Electrophoretic separation of rat skeletal muscle myosin heavy chain isoforms*
**作者**：Talmadge, R.J., et al.
**摘要**：提出一种结合SDS-PAGE电泳和MyHC抗体的方法，高效分离并鉴定大鼠骨骼肌中四种MyHC亚型，为肌肉代谢和运动适应性研究提供技术基础。
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**备注**：以上文献发表于1989-2015年间，覆盖基础机制、疾病模型和技术方法，MyHC抗体在其中均作为关键工具用于蛋白定位、分型或功能研究。
Myosin heavy chain (MHC) antibodies are essential tools in studying the structure, function, and diversity of myosin proteins, which are critical components of muscle contraction. Myosin is a motor protein composed of heavy and light chains, with the heavy chain determining its isoform-specific properties. MHC exists in multiple isoforms encoded by distinct genes, including slow-twitch (Type I, e.g., MHC-β/Myh7) and fast-twitch (Type IIa/Myh2. IIx/Myh1. IIb/Myh4) variants in skeletal muscle, as well as cardiac-specific isoforms (e.g., α-MHC/Myh6 and β-MHC/Myh7). These isoforms dictate contractile speed, metabolic efficiency, and functional specialization across muscle types.
MHC antibodies are widely used to identify muscle fiber types in research on skeletal muscle plasticity, cardiac hypertrophy, and neuromuscular disorders. For example, antibodies targeting MHC-I highlight slow, oxidative fibers involved in endurance, while those against MHC-II detect fast, glycolytic fibers associated with explosive movements. In cardiac research, shifts between α- and β-MHC isoforms serve as biomarkers for pathological remodeling. Additionally, MHC antibodies aid in diagnosing myopathies, monitoring muscle regeneration (via embryonic/neonatal MHC isoforms), and studying evolutionary conservation across species. Their specificity enables precise localization in techniques like immunohistochemistry, Western blotting, and flow cytometry. Commercial availability and well-characterized epitopes make MHC antibodies indispensable in muscle physiology, disease modeling, and therapeutic development.
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