| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNAP23 |
| Uniprot No | O00161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MDNLSSEEIQQRAHQITDESLESTRRILGLAIESQDAGIKTITMLDEQKEQLNRIEEGLDQINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANARAKKLIDS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis"**
- **作者**: Suzuki H. et al.
- **摘要**: 研究通过基因敲除模型揭示了SNAP23在血小板和肥大细胞发育中的关键作用，并通过重组蛋白实验验证了其与SNARE复合物互作在细胞分泌中的功能。
2. **"Recombinant SNAP23 binds multiple syntaxin isoforms and participates in vesicle trafficking in vitro"**
- **作者**: Scales S.J. et al.
- **摘要**: 利用重组SNAP23蛋白进行体外结合实验，证明其与多种Syntaxin蛋白(如Syntaxin-1A/4)相互作用，并调控囊泡运输的分子机制。
3. **"Structural and functional analysis of SNAP23 in regulated exocytosis"**
- **作者**: Paumet F. et al.
- **摘要**: 通过重组人源SNAP23蛋白的纯化和结构分析，阐明了其C端结构域在胞吐过程中与VAMP8和Syntaxin-4形成功能性SNARE复合物的机制。
4. **"A role for SNAP23 in early phases of glucose-stimulated insulin secretion from pancreatic β-cells"**
- **作者**: Spurlin B.A. et al.
- **摘要**: 利用重组SNAP23蛋白和β细胞模型，揭示了SNAP23通过调控胰岛素囊泡与质膜融合的早期阶段，影响葡萄糖刺激的胰岛素分泌过程。
**Background of SNAP23 Recombinant Protein**
SNAP23 (Synaptosome-Associated Protein 23 kDa) is a member of the SNARE (Soluble NSF Attachment Protein Receptor) family, which plays a central role in intracellular membrane fusion events. Unlike its neuronal counterpart SNAP25. SNAP23 is ubiquitously expressed and mediates vesicle trafficking in non-neuronal cells, including exocytosis, endocytosis, and organelle membrane fusion. It functions by forming a stable ternary complex with syntaxin and VAMP (vesicle-associated membrane protein) family members, facilitating the docking and fusion of vesicles to target membranes.
Recombinant SNAP23 protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional SNAP23 for research. Its recombinant form allows precise study of SNARE complex assembly, membrane fusion mechanisms, and interactions with regulatory proteins like NSF (N-ethylmaleimide-sensitive factor) or α-SNAP. Researchers utilize it to investigate pathologies linked to SNARE dysfunction, such as endocrine disorders, immune response defects, and cancer metastasis, where aberrant vesicle trafficking contributes to disease progression.
In drug discovery, recombinant SNAP23 serves as a tool to screen molecules modulating membrane fusion, potentially targeting secretion-related diseases. Structural studies using the protein have clarified its domain organization, including two SNARE motifs and a palmitoylation site critical for membrane localization. Despite its importance, SNAP23's regulatory mechanisms and tissue-specific roles remain active areas of study, driving demand for high-purity recombinant variants to advance cellular and therapeutic research.
×
