| 纯度 | ＞80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNAI2 |
| Uniprot No | O43623 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMPRSFLVKKHFNASKKPNYSELDTHTV IISPYLYESYSMPVIPQPEILSSGAYSPITVWTTAAPFHAQLPNGLSPLS GYSSSLGRVSPPPPSDTSSKDHSGSESPISDEEERLQSKLSDPHAIEAEK FQCNLCNKTYSTFSGLAKHKQLHCDAQSRKSFSCKYCDKEYVSLGALKMH IRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSN LRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGCCVAH |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNAI2重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
---
1. **文献名称**：*"Recombinant SNAI2 Protein Induces Epithelial-Mesenchymal Transition in Mammary Epithelial Cells"*
**作者**：Smith J, et al.
**摘要**：本研究在大肠杆菌中表达并纯化了重组人源SNAI2蛋白，验证其通过下调E-cadherin表达诱导乳腺上皮细胞发生上皮间质转化(EMT)的能力，证实其在肿瘤转移中的作用。
2. **文献名称**：*"Purification and Functional Analysis of SNAI2/Slug Recombinant Protein in Neural Crest Development"*
**作者**：Lee C, Kim D.
**摘要**：报道了利用昆虫杆状病毒系统制备高活性SNAI2重组蛋白，通过体外实验证明其通过结合靶基因启动子调控神经嵴细胞迁移及分化。
3. **文献名称**：*"Structural Characterization of SNAI2 Zinc Finger Domain by NMR Using Recombinant Protein"*
**作者**：Zhang R, et al.
**摘要**：通过NMR技术解析了重组SNAI2蛋白锌指结构域的三维结构，揭示了其与DNA结合的关键氨基酸残基，为设计靶向抑制剂提供结构基础。
---
说明：上述文献为示例，实际引用需根据具体研究内容检索PubMed或Web of Science等数据库。建议使用关键词“SNAI2 recombinant protein expression”或“SNAI2 purification”进一步筛选。
SNAI2 (Snail Family Transcriptional Repressor 2), also known as Slug, is a member of the Snail superfamily of zinc-finger transcription factors. It plays a critical role in embryonic development, particularly in epithelial-mesenchymal transition (EMT), a process essential for cell migration, tissue remodeling, and cancer metastasis. SNAI2 regulates gene expression by binding to E-box sequences in target gene promoters, often repressing epithelial markers like E-cadherin while promoting mesenchymal traits. Dysregulation of SNAI2 is linked to tumor progression, invasiveness, and drug resistance in various cancers.
Recombinant SNAI2 protein is engineered for experimental studies to dissect its molecular functions. Typically produced in *E. coli* or mammalian expression systems, the protein is purified using affinity tags (e.g., His-tag) for high purity. Its structure includes conserved C-terminal zinc-finger domains critical for DNA binding and N-terminal repression domains. Researchers utilize recombinant SNAI2 to study EMT mechanisms, screen inhibitors targeting metastatic pathways, or analyze protein-DNA/ protein-protein interactions. It also serves as an antigen for antibody development in diagnostic assays. Despite challenges in maintaining its native conformation *in vitro*, recombinant SNAI2 remains a vital tool for exploring cancer biology and developmental processes.
×
