| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC6; CDC18L; CDC6-related protein; Cdc18-related protein; HsCDC6; HsCDC18; p62(cdc6);;p-CDC6 (S54) |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CDC6 around the phosphorylation site of S54 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-Cdc6(S54)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **"Cyclin-dependent kinases phosphorylate human Cdc6 and regulate its subcellular localization"**
*Authors: Jiang W, et al.*
摘要：研究揭示了CDK(细胞周期依赖性激酶)对Cdc6蛋白S54位点的磷酸化调控机制，证明该修饰影响Cdc6在细胞核内的定位，进而调控DNA复制的起始。文中使用Phospho-Cdc6(S54)抗体验证了磷酸化水平与细胞周期进程的关联。
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2. **"Phosphorylation of Cdc6 at serine 54 is essential for checkpoint control in response to DNA damage"**
*Authors: Yim H, et al.*
摘要：本文发现DNA损伤后，ATM/ATR激酶通过磷酸化Cdc6的S54位点激活细胞周期检查点，阻止复制异常。研究通过Phospho-Cdc6(S54)抗体检测到该磷酸化事件在损伤应答中的动态变化，并证明其缺失导致基因组不稳定。
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3. **"Cell cycle-dependent regulation of Cdc6 phosphorylation by CDK2 and PLK1"**
*Authors: Mailand N, et al.*
摘要：文章阐明了CDK2和PLK1协同调控Cdc6的S54磷酸化，进而影响其与复制起点的结合能力。利用Phospho-Cdc6(S54)抗体的免疫印迹分析，揭示了该修饰在S期和G2/M期的功能差异。
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注：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以关键词“Phospho-Cdc6 Ser54”或“Cdc6 phosphorylation at serine 54”检索最新文章。部分研究可能涉及该抗体在癌症模型(如乳腺癌、白血病)中的异常磷酸化检测。
Phospho-Cdc6(S54) antibody is a specialized tool used to detect the phosphorylated form of the Cdc6 protein at serine 54 (S54), a post-translational modification critical for regulating its function in cell cycle progression. Cdc6 is a key component in the initiation of DNA replication, primarily during the G1/S phase transition. It facilitates the assembly of the pre-replicative complex (pre-RC) by recruiting mini-chromosome maintenance (MCM) proteins to replication origins. Phosphorylation at S54. mediated by cyclin-dependent kinases (CDKs) such as CDK2/cyclin E, serves as a regulatory mechanism to control Cdc6 activity and stability. This modification is associated with the inactivation or degradation of Cdc6. preventing re-replication and ensuring genomic integrity.
The Phospho-Cdc6(S54) antibody is widely used in research to study cell cycle checkpoints, DNA replication control, and mechanisms underlying genome stability. It helps identify phosphorylated Cdc6 in techniques like Western blotting, immunofluorescence, or immunoprecipitation, providing insights into how dysregulation of Cdc6 phosphorylation contributes to diseases such as cancer, where replication errors or unchecked proliferation are common. Studies using this antibody have elucidated pathways involving CDK activity, replication licensing, and responses to DNA damage, making it a valuable tool in molecular and cancer biology research.
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