| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; Mitogen activated protein kinase kinase kinase 3;;MEKK3 |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEKK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于MEKK3抗体的示例文献(注：文献为示例性虚构，实际引用需核实原文)：
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1. **文献名称**: *"MEKK3 regulates TLR4-induced NF-κB activation through phosphorylation of IKK complex"*
**作者**: Yang J, et al.
**摘要**: 本研究利用MEKK3特异性抗体证实，MEKK3通过磷酸化IKKα/β调控TLR4信号通路中的NF-κB激活，敲除MEKK3显著抑制巨噬细胞的炎症因子释放。
2. **文献名称**: *"MEKK3 promotes tumor progression via ERK1/2 signaling in triple-negative breast cancer"*
**作者**: Li X, et al.
**摘要**: 通过免疫印迹(MEKK3抗体)和功能实验发现，MEKK3在乳腺癌中高表达，通过激活ERK1/2通路促进肿瘤细胞侵袭和转移，提示其作为潜在治疗靶点。
3. **文献名称**: *"MEKK3 deficiency disrupts embryonic angiogenesis by impairing VEGF signaling"*
**作者**: Wang R, et al.
**摘要**: 研究利用MEKK3敲除小鼠及抗体检测，发现MEKK3缺失导致胚胎血管发育异常，机制涉及VEGF受体下游的MAPK信号通路失调。
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**提示**：以上文献为示例，实际引用请通过PubMed或Web of Science检索真实文献(关键词：MEKK3 antibody, MAP3K3. signaling pathway)。
The MEKK3 (Mitogen-Activated Protein Kinase Kinase Kinase 3) antibody is a critical tool for studying the MEKK3 protein, a serine/threonine kinase within the MAP3K family. MEKK3 plays a pivotal role in regulating multiple signaling pathways, including the NF-κB, ERK, and p38 MAPK cascades, which are involved in cellular responses to stress, inflammation, proliferation, and apoptosis. It acts upstream of downstream kinases, mediating signals from cell surface receptors to intracellular targets. Dysregulation of MEKK3 has been implicated in various pathologies, such as cardiovascular diseases, cancer, and immune disorders, making it a focus of biomedical research.
MEKK3 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect MEKK3 expression, localization, and post-translational modifications. These antibodies help elucidate MEKK3’s interaction partners, activation mechanisms, and tissue-specific roles. For instance, studies have revealed MEKK3’s involvement in angiogenesis, embryonic development, and cytokine signaling. Researchers often validate antibody specificity using knockout cell lines or siRNA-mediated MEKK3 depletion to ensure accurate results.
Commercially available MEKK3 antibodies are typically raised against specific epitopes, such as the N-terminal or kinase domains, and vary in host species (e.g., rabbit, mouse). Selecting the right antibody depends on the experimental application and species reactivity. Proper controls and optimization are essential due to potential cross-reactivity with homologous kinases like MEKK2. Overall, MEKK3 antibodies are indispensable for advancing our understanding of cellular signaling networks and their implications in health and disease.
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