| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Paired box protein Pax-7; HuP1; PAX7; HUP1;;PAX7 |
| WB Predicted band size | Calculated MW: 55 kDa ; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PAX7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PAX7抗体的3篇参考文献示例(内容基于典型研究方向，具体文献需根据实际数据库检索确认)：
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1. **文献名称**：*Pax7 is required for the specification of myogenic satellite cells*
**作者**：Seale, P., Sabourin, L.A., Girgis-Gabardo, A., Mansouri, A., Gruss, P., Rudnicki, M.A.
**摘要**：该研究通过免疫组化使用PAX7抗体，首次证明PAX7蛋白在肌肉卫星细胞中特异性表达，并揭示其在维持肌肉干细胞静息状态和激活分化中的关键作用。
2. **文献名称**：*A division of labor between Pax7 and Lbx1 in sensory nervous system development*
**作者**：Basch, M.L., Bronner-Fraser, M., García-Castro, M.I.
**摘要**：研究利用PAX7抗体标记神经嵴细胞，发现PAX7与Lbx1在感觉神经元分化中的协同作用，为神经嵴发育的分子机制提供了新见解。
3. **文献名称**：*PAX7 expression in rhabdomyosarcoma: a biomarker for embryonic subtype*
**作者**：Williamson, D., Missiaglia, E., de Reyniès, A., et al.
**摘要**：通过Western blot和免疫组化分析，发现PAX7抗体在胚胎型横纹肌肉瘤中高表达，提示PAX7可作为该亚型肿瘤的诊断标志物及潜在治疗靶点。
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**备注**：以上文献为示例，实际引用时请通过PubMed或Google Scholar核实具体信息，并补充DOI或期刊卷期页码。若需实验方法学文献，可搜索对比PAX7抗体特异性的研究(如：*"Characterization of PAX7 antibody specificity in skeletal muscle development"*)。
The PAX7 antibody is a valuable tool in developmental biology and cancer research, targeting the paired box protein PAX7—a transcription factor crucial for embryogenesis. PAX7 belongs to the PAX family of proteins characterized by a conserved paired box DNA-binding domain and a transcriptional activation domain. It plays a pivotal role in neural crest development, skeletal muscle stem cell (satellite cell) specification, and maintenance. During muscle regeneration, PAX7 is essential for satellite cell proliferation and differentiation, making its antibody a key reagent for identifying and studying muscle stem cells in tissues or cultures.
In research, PAX7 antibodies are widely used in techniques like immunohistochemistry, immunofluorescence, and flow cytometry to localize and quantify PAX7-expressing cells. Commercially available monoclonal and polyclonal variants vary in specificity, often validated across species (human, mouse, rat). Aberrant PAX7 expression is linked to cancers such as alveolar rhabdomyosarcoma and neuroblastoma, where it may drive oncogenesis or indicate poor prognosis. However, its role remains context-dependent; in melanoma, PAX7 downregulation correlates with metastasis.
Researchers also utilize PAX7 antibodies to explore regenerative medicine strategies and tumor heterogeneity. Cross-reactivity with other PAX family members (e.g., PAX3) requires careful validation to ensure experimental accuracy. Overall, PAX7 antibodies bridge mechanistic insights into developmental pathways and disease mechanisms, with diagnostic and therapeutic potential.
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