| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5''-AMP-activated protein kinase subunit beta-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; HAMPKb; PRKAB1;;AMPK beta 1 |
| WB Predicted band size | Calculated MW: 30 kDa ; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human AMPK beta 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及AMPKβ1抗体的参考文献及其摘要概括：
1. **"AMPK β1 deficiency reduces glucose tolerance and induces β-cell dysfunction in mice"**
- **作者**: Sakamoto K. et al.
- **摘要**: 研究利用AMPKβ1基因敲除小鼠模型，通过Western blot和免疫组化分析发现，AMPKβ1缺失导致胰岛素分泌受损及β细胞功能异常，揭示了AMPKβ1在葡萄糖代谢中的关键作用。
2. **"Differential regulation of AMPK β-subunit isoforms in skeletal muscle by chronic energy deprivation"**
- **作者**: O'Neill H.M. et al.
- **摘要**: 通过AMPKβ1特异性抗体分析发现，长期能量限制(如禁食)显著上调骨骼肌中AMPKβ1蛋白表达，提示其亚型在能量应激下的特异性调控机制。
3. **"AMPK β1 localization in lipid droplets regulates fatty acid metabolism"**
- **作者**: Liu Y. et al.
- **摘要**: 利用免疫荧光和共聚焦显微镜技术，发现AMPKβ1定位于脂滴，并通过抗体阻断实验证实其参与脂肪酸氧化调控，为代谢疾病治疗提供新靶点。
以上文献展示了AMPKβ1抗体在基因功能、亚细胞定位及代谢调控研究中的应用。
AMPK beta1 antibody is a key tool used to study the beta1 regulatory subunit of AMP-activated protein kinase (AMPK), a crucial energy sensor in eukaryotic cells. AMPK is a heterotrimeric enzyme composed of α (catalytic), β, and γ subunits, with β subunits (beta1 and beta2) serving structural roles by anchoring the complex and regulating enzyme activity. The beta1 subunit (encoded by PRKAB1) is ubiquitously expressed and facilitates AMPK assembly, stability, and interaction with glycogen via its carbohydrate-binding module.
Antibodies targeting AMPK beta1 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions in tissues or cells. They help investigate AMPK's role in metabolic regulation, including glucose uptake, lipid metabolism, and mitochondrial homeostasis. Researchers also employ these antibodies to study AMPK dysregulation in diseases like diabetes, obesity, cancer, and neurodegenerative disorders.
Specificity is critical, as beta1 shares homology with beta2; thus, high-quality antibodies are validated to minimize cross-reactivity. These reagents are essential for elucidating isoform-specific functions and signaling pathways, particularly in tissue-specific knockout models. Commercial AMPK beta1 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins, with validation in knockdown/knockout controls. Their application supports translational research aiming to develop AMPK-targeted therapies for metabolic and age-related diseases.
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