| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A;;FOXO3A |
| WB Predicted band size | Calculated MW: 71 kDa ; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FOXO3A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于FOXO3A抗体的3篇参考文献(均为虚构示例，实际文献需根据真实数据库检索)：
1. **文献名称**: *"FOXO3A modulates oxidative stress resistance through transcriptional regulation of antioxidant genes"*
**作者**: Brunet A., et al.
**摘要**: 该研究利用FOXO3A特异性抗体，通过ChIP实验和Western blot分析，揭示了FOXO3A在氧化应激条件下直接调控超氧化物歧化酶(SOD2)和过氧化氢酶的表达，从而增强细胞抗氧化能力。
2. **文献名称**: *"Phosphorylation-dependent regulation of FOXO3A nuclear localization in cancer cells"*
**作者**: Myatt S.S., Lam E.W.
**摘要**: 作者采用FOXO3A抗体进行免疫荧光和亚细胞分馏实验，证明AKT介导的FOXO3A磷酸化可促进其胞质滞留，抑制其转录活性，进而影响肿瘤细胞的增殖与凋亡。
3. **文献名称**: *"Validation of FOXO3A antibody specificity for human tissue immunohistochemistry"*
**作者**: Huang H., et al.
**摘要**: 本研究系统验证了多种FOXO3A抗体的特异性，通过siRNA敲低和过表达模型，确认了某商业抗体在人类组织切片中检测FOXO3A蛋白的可靠性，为临床病理研究提供工具支持。
**提示**: 实际文献需通过PubMed、Google Scholar等平台以关键词“FOXO3A antibody”、“FOXO3A validation”检索，并筛选涉及抗体应用的基础或方法学论文。
The FOXO3A antibody is a crucial tool in studying the FOXO3A protein, a member of the Forkhead box O (FOXO) family of transcription factors. FOXO3A regulates genes involved in cell cycle arrest, apoptosis, oxidative stress resistance, and longevity. It acts as a tumor suppressor and is tightly regulated by post-translational modifications (e.g., phosphorylation, acetylation) that influence its subcellular localization (nucleus vs. cytoplasm) and activity. Dysregulation of FOXO3A is linked to cancer, neurodegenerative diseases, and aging-related processes, making it a focal point in biomedical research.
FOXO3A antibodies are typically generated using immunogens such as recombinant FOXO3A protein fragments or synthetic peptides corresponding to specific epitopes. These antibodies are available in monoclonal or polyclonal forms, often produced in hosts like rabbits or mice. They are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect FOXO3A expression, localization, and interactions. Specific antibodies may target distinct regions (e.g., N-terminal, C-terminal) or modified states (e.g., phosphorylated FOXO3A at Ser318/321) to study its regulatory mechanisms.
Validation of FOXO3A antibodies is critical, involving knockout cell lines or siRNA-mediated knockdown to confirm specificity. High-quality antibodies enable researchers to explore FOXO3A's role in signaling pathways (e.g., PI3K/AKT, AMPK) and its downstream targets (e.g., p27kip1. BIM). Such studies provide insights into therapeutic strategies for diseases associated with FOXO3A dysfunction.
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