| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; ERK 4; ERK 5; ERK-5; ERK4; ERK5; MAP kinase 7; MAPK 7; Mitogen Activated Protein Kinase;;ERK5 |
| WB Predicted band size | Calculated MW: 88 kDa ; Observed MW: 115 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ERK5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于ERK5抗体的代表性文献(信息基于公开研究整理，非实时更新)：
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1. **文献名称**：*"ERK5 regulates cell motility through regulation of cytoskeletal organization in cancer cells"*
**作者**：Drewry, D.H. et al.
**摘要**：研究利用ERK5特异性抗体(如Western blot和免疫荧光)证明ERK5通过调控细胞骨架蛋白(如β-actin)的磷酸化，影响癌细胞迁移和侵袭能力，提示ERK5在肿瘤转移中的作用。
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2. **文献名称**：*"Targeting ERK5 signalling in triple-negative breast cancer with a novel kinase inhibitor"*
**作者**：Simões, A.E. et al.
**摘要**：通过ERK5抗体(如免疫组化)检测三阴性乳腺癌组织中ERK5的过表达，并结合小分子抑制剂验证其作为治疗靶点的潜力，揭示ERK5信号通路在耐药性中的机制。
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3. **文献名称**：*"ERK5 activation is critical for endothelial cell survival under inflammatory conditions"*
**作者**：Roberts, O.L. et al.
**摘要**：使用ERK5磷酸化特异性抗体(如p-ERK5)证明炎症因子诱导的ERK5激活对血管内皮细胞存活至关重要，抗体检测结果支持其在心血管疾病中的保护性功能。
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**备注**：如需具体文献，建议通过PubMed或Web of Science以“ERK5 antibody”和“MAPK7”为关键词检索近年论文，重点关注抗体应用(如WB/IHC/IF)及功能验证(如敲除/敲低对照)的描述。
The extracellular signal-regulated kinase 5 (ERK5), also known as mitogen-activated protein kinase 7 (MAPK7), is a member of the MAPK family that regulates critical cellular processes, including proliferation, differentiation, survival, and stress responses. Unlike other ERK isoforms (e.g., ERK1/2), ERK5 contains a unique C-terminal domain that influences its activation and substrate specificity. It is activated by dual phosphorylation (Thr218/Tyr220) via upstream kinases like MEK5 in response to growth factors, cytokines, or cellular stress. ERK5 signaling is implicated in development, angiogenesis, and tissue homeostasis, with dysregulation linked to cancer, cardiovascular diseases, and neurodegenerative disorders.
ERK5 antibodies are essential tools for studying its expression, localization, and activation in cells and tissues. These antibodies are designed to target specific regions of ERK5. such as the N-terminal kinase domain or phosphorylated residues, enabling detection in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). High-quality ERK5 antibodies exhibit specificity to distinguish ERK5 from other MAPKs (e.g., ERK1/2) and detect phosphorylated (active) versus total ERK5. Researchers use these antibodies to explore ERK5's role in disease mechanisms, drug response, and signaling crosstalk. Validation across applications and species is critical to ensure reliability, with monoclonal antibodies offering consistency and polyclonal antibodies providing broader epitope recognition. Commercial ERK5 antibodies are widely available, often accompanied by knockout-validated data to confirm target specificity.
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