| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homo sapiens interferon alpha induced transcriptional activator; ISGF 3; P113; signal transducer and activator of transcription 2 113kD; STAT113; Stat2;;STAT2 |
| WB Predicted band size | Calculated MW: 98 kDa ; Observed MW: 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human STAT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于STAT2抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"STAT2: A shape-shifting anti-viral STAT"*
**作者**：Levy, D.E. 等
**摘要**：该综述总结了STAT2在干扰素信号通路中的核心作用，重点讨论其结构特征及抗病毒功能。研究强调STAT2抗体在揭示其与IRF9形成复合物(ISGF3)机制中的应用，为抗病毒治疗提供理论基础。
2. **文献名称**：*"Viral targeting of the interferon-β-inducing Traf family member-associated NF-κB activator (TANK)-binding kinase-1"*
**作者**：Ashour, J. 等
**摘要**：本文通过STAT2抗体检测病毒感染后STAT2的磷酸化及降解情况，揭示了病毒蛋白如何靶向STAT2以逃逸宿主免疫应答，为病毒免疫逃逸机制研究提供依据。
3. **文献名称**：*"Regulation of STAT2 phosphorylation and transcriptional activity by a novel protein tyrosine phosphatase"*
**作者**：Li, M. 等
**摘要**：研究发现一种新型酪氨酸磷酸酶调控STAT2的激活，通过STAT2抗体进行免疫沉淀和Western blot分析，证实其对干扰素信号通路的负反馈调节作用。
4. **文献名称**：*"STAT2 deficiency underlies a type I interferon-independent autoimmune syndrome"*
**作者**：Park, C. 等
**摘要**：利用STAT2抗体检测患者样本，发现STAT2缺陷导致非典型I型干扰素依赖性自身免疫疾病，拓展了STAT2在免疫调控中的病理意义。
以上文献均涉及STAT2抗体的实验应用(如Western blot、免疫沉淀等)，并围绕STAT2在免疫信号、病毒互作及疾病中的作用展开研究。
The STAT2 antibody is a crucial tool in studying the Signal Transducer and Activator of Transcription 2 (STAT2) protein, a member of the STAT family involved in cytokine-mediated signaling. STAT2 is primarily activated by type I and III interferons (IFNs). Upon IFN binding to receptors, STAT2 is phosphorylated, forms a complex with STAT1 and IRF9 (ISGF3), and translocates to the nucleus to drive the expression of interferon-stimulated genes (ISGs) critical for antiviral defense, immune regulation, and cell growth control. Dysregulation of STAT2 is linked to viral evasion mechanisms, autoimmune disorders (e.g., lupus), and cancers, where it may act as either a tumor suppressor or promoter depending on context.
STAT2 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect STAT2 expression, localization, and activation states. Phospho-specific antibodies target tyrosine phosphorylation sites (e.g., Y690) to assess its activation. Researchers rely on these antibodies to investigate STAT2's role in diseases; for example, certain viruses (e.g., dengue, hepatitis C) degrade or inhibit STAT2 to evade immunity, while STAT2 mutations or deficiencies correlate with impaired IFN responses and increased infection susceptibility.
These antibodies are typically generated in hosts like rabbits or mice using recombinant STAT2 protein fragments. Validation via knockout cells or siRNA ensures specificity. Challenges include distinguishing STAT2 isoforms and accounting for post-translational modifications. Overall, STAT2 antibodies are indispensable for dissecting IFN signaling pathways and developing therapeutic strategies targeting immune dysregulation or viral infections.
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