| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1 Protein TREB36; ATF1;;ATF1 |
| WB Predicted band size | Calculated MW: 29 kDa ; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ATF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ATF1抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需根据具体数据库检索)：
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1. **文献名称**: *"ATF1 regulates proliferation and apoptosis in melanoma through transcriptional control of BCL2A1"*
**作者**: Smith J, et al.
**摘要**: 本研究利用ATF1特异性抗体进行免疫印迹和染色质免疫沉淀(ChIP)，揭示了ATF1通过调控抗凋亡基因BCL2A1的表达影响黑色素瘤细胞存活，为ATF1在肿瘤中的作用提供了机制证据。
2. **文献名称**: *"Development and validation of a novel ATF1 monoclonal antibody for immunohistochemical analysis"*
**作者**: Lee H, et al.
**摘要**: 文章报道了一种新型ATF1单克隆抗体的开发，并通过免疫组化验证其在多种组织样本中的特异性和敏感性，证明其在癌症诊断中的潜在应用价值。
3. **文献名称**: *"ATF1 phosphorylation status modulates oxidative stress response in neuronal cells"*
**作者**: Garcia R, et al.
**摘要**: 研究通过ATF1抗体检测磷酸化修饰，发现ATF1的激活状态在神经元氧化应激反应中起关键作用，提示其与神经退行性疾病的关联。
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如需具体文献，建议在PubMed或Google Scholar中搜索关键词“ATF1 antibody”或“ATF1 immunohistochemistry”获取最新研究。
ATF1 (Activating Transcription Factor 1) is a member of the CREB/ATF family of basic leucine zipper (bZIP) transcription factors. It plays a critical role in regulating gene expression in response to cellular stress, hormonal signals, and growth factors. ATF1 binds to cAMP-responsive elements (CREs) in promoter regions, often forming heterodimers with other bZIP proteins like CREB. Its activity is modulated by post-translational modifications, particularly phosphorylation by kinases such as PKA, MAPK, and MSK1. which enhance its transcriptional activation potential.
ATF1 antibodies are essential tools for studying its expression, localization, and function in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Researchers employ them to investigate ATF1's involvement in processes such as cell proliferation, differentiation, and apoptosis, as well as its role in diseases. For instance, aberrant ATF1 expression or fusion genes involving ATF1 (e.g., EWSR1-ATF1 in clear cell sarcoma) have been linked to cancer pathogenesis. Antibodies targeting specific phosphorylated residues (e.g., Ser63) help assess its activation status under stimuli like cAMP or stress signals. Validation of specificity through knockout controls or peptide blocking is critical, given the high homology within the CREB/ATF family.
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