| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIRS1; Insulin receptor substrate 1; IRS 1; IRS1;;IRS 1 |
| WB Predicted band size | Calculated MW: 132 kDa ; Observed MW: 170 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human IRS 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于IRS1抗体的3篇参考文献及其摘要概述：
1. **文献名称**: "Role of IRS-1 tyrosine phosphorylation in insulin resistance"
**作者**: Sun, X.J., et al.
**摘要**: 研究使用IRS1抗体检测胰岛素刺激下IRS1酪氨酸磷酸化水平，发现2型糖尿病患者肌肉组织中磷酸化降低，提示其与胰岛素信号传导受损相关。
2. **文献名称**: "IRS-1 regulates prostate cancer cell growth through AKT activation"
**作者**: Draznin, B., et al.
**摘要**: 通过IRS1抗体进行Western blot和免疫组化分析，证实前列腺癌细胞中IRS1过表达激活AKT通路，促进肿瘤增殖，靶向IRS1可抑制体内外肿瘤生长。
3. **文献名称**: "Tissue-specific knockout of IRS-1 alters glucose metabolism in liver and muscle"
**作者**: Kubota, N., et al.
**摘要**: 利用IRS1抗体验证肝脏和肌肉特异性敲除小鼠模型，发现肝脏IRS1缺失导致糖异生异常，而肌肉缺失引起葡萄糖摄取障碍，揭示组织特异性代谢调控机制。
以上研究均通过IRS1抗体进行蛋白表达或修饰分析，涵盖代谢疾病、肿瘤及分子机制领域。
The insulin receptor substrate 1 (IRS1) antibody is a crucial tool in studying insulin signaling and metabolic regulation. IRS1. a cytoplasmic adaptor protein, mediates signaling from the insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R). Upon receptor activation, IRS1 undergoes tyrosine phosphorylation, enabling interactions with downstream effectors like PI3K and GRB2. which regulate glucose uptake, cell growth, and metabolism. Dysregulation of IRS1 is linked to insulin resistance, type 2 diabetes, and certain cancers.
IRS1 antibodies are widely used in research to detect IRS1 expression, phosphorylation status, and protein interactions via techniques such as Western blotting, immunohistochemistry, and immunoprecipitation. They help elucidate mechanisms underlying metabolic disorders and cancer progression. For example, reduced IRS1 tyrosine phosphorylation or increased serine phosphorylation (associated with insulin resistance) can be quantified using phospho-specific antibodies.
Commercial IRS1 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal, C-terminal, or phosphorylated residues). Validation includes testing in knockout models or siRNA-treated cells to ensure specificity. Beyond research, IRS1 antibodies hold potential in diagnostic assays for metabolic diseases and therapeutic development, particularly in targeting insulin resistance pathways. Their application continues to advance understanding of cellular signaling networks in health and disease.
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