首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子免疫球蛋白M(IgM)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgM含量。IgM是人体免疫系统中重要的免疫球蛋白。在它是个体发育过程中最早合成和分泌的抗体。其研究机制主要聚焦在免疫识别与防御，可快速结合病原体激活补体系统，在抗感染初期发挥关键作用，也在自身免疫病等方面有重要研究价值。试剂盒检测范围为15.6 ng/mL-4000 ng/mL，在评估实验动物免疫状态或感染模型建立等科研场景中广泛应用；适用于疫苗开发、感染性疾病动物模型研究、免疫抑制剂效果评估等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgM
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：15.6 ng/mL-4000 ng/mL
灵敏度：4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 96 | |||||
| Range % | 87-105 | ||||||
| 1:2000 | Average % | 92 | |||||
| Range % | 85-100 | ||||||
| 1:4000 | Average % | 93 | |||||
| Range % | 89-100 | ||||||
| 1:8000 | Average % | 94 | |||||
| Range % | 85-99 | ||||||
回收率：
| The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 93 | 87-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 40000.2250.2180.222 10000.3510.3450.348 2500.6230.6170.62 62.51.1271.1391.133 15.62.2262.2412.234 02.9112.9842.948 | |||||||
货期：3-5 working days
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