| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; GluRA; GluRK1; Glutamate receptor ionotropic, AMPA 1; GluA1; GRIA1; GLUH1; GLUR1;;GluR1 |
| WB Predicted band size | 102 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GluR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与GluR1抗体相关的经典文献及其摘要概述，供参考：
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1. **文献名称**：*Regulation of AMPA receptor trafficking by synaptic signaling*
**作者**：Derkach VA, Oh MC, Guire ES, Soderling TR (2007)
**摘要**：研究报道了GluR1抗体在检测神经元表面AMPA受体动态分布中的应用，揭示了磷酸化位点特异性抗体如何揭示突触可塑性中GluR1的亚细胞定位变化。
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2. **文献名称**：*AMPA receptor trafficking and synaptic plasticity*
**作者**：Malinow R, Malenka RC (2002)
**摘要**：该综述总结了GluR1抗体在Western blot和免疫组化中的关键作用，强调了其在研究LTP(长时程增强)过程中AMPA受体膜插入机制的应用。
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3. **文献名称**：*GluR1-dependent synaptic plasticity is critical for fear memory*
**作者**：Lu W, Shi Y, Malenka RC (2009)
**摘要**：通过GluR1抗体的特异性标记，研究证明GluR1在杏仁核突触中的表达上调与恐惧记忆形成密切相关，抗体数据支持了行为学与分子机制的关联。
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4. **文献名称**：*Subunit-specific rules governing AMPA receptor trafficking to synapses*
**作者**：Shepherd JD, Huganir RL (2006)
**摘要**：利用GluR1亚基特异性抗体，研究揭示了神经元活动依赖性运输中GluR1与GluR2的差异化调控机制，为抗体在区分受体亚型功能提供了范例。
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这些文献均以GluR1抗体为工具，聚焦于其在突触可塑性、记忆或受体运输机制研究中的应用。如需具体实验细节或抗体货号，建议进一步查阅原文方法学部分。
The GluR1 antibody is a crucial tool in neuroscience research, targeting the GluR1 subunit (also known as GluA1) of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor. AMPA receptors are ionotropic glutamate receptors that mediate fast excitatory synaptic transmission in the central nervous system. The GluR1 subunit plays a pivotal role in synaptic plasticity, learning, and memory by regulating receptor trafficking and membrane insertion. Structurally, GluR1 comprises four transmembrane domains with intracellular N- and C-termini, forming a tetrameric ion channel. Its expression and phosphorylation states (e.g., at Ser845 or Ser831) influence synaptic strength and long-term potentiation (LTP).
GluR1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study protein expression, localization, and post-translational modifications. They are essential for investigating neurological disorders, such as Alzheimer’s disease, epilepsy, and stroke, where AMPA receptor dysregulation is implicated. Both monoclonal and polyclonal variants exist, with specificity validated through knockout controls or peptide-blocking assays. Researchers must optimize experimental conditions (e.g., detergent use for membrane protein extraction) and consider epitope regions to avoid cross-reactivity with homologous subunits (GluR2-4). Commercial antibodies often specify host species, clonality, and recommended applications, ensuring reliability across studies. Proper handling, including tissue fixation and storage, is critical to preserve GluR1’s conformational integrity for accurate detection.
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